大鼠脾切除对移植脂肪间充质干细胞的肝脏归巢效率及肝硬化的影响  

Effects of splenectomy on liver homing and cirrhosis therapy after transplantation with adipose tissue derived mesenchymal stem cells in rats

作　　者：唐卫平 李翀[1] 陈国栋[1] 赵磊[2] TANG Wei-ping;LI Chong;CHEN Guo-dong;ZHAO Lei(Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital,University of South China,Hengyang 421001,China;Department of Gastrointestinal Surgery,the First Affiliated Hospital,University of South China, Hengyang 421001, China)

机构地区：[1]南华大学附属第一医院肝胆胰外科,湖南衡阳421001 [2]南华大学附属第一医院胃肠外科,湖南衡阳421001

出　　处：《基础医学与临床》2021年第10期1428-1433,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金青年科学项目(81900488)。

摘　　要：目的探讨脾切除对移植脂肪间充质干细胞(ADSCs)的肝脏归巢及肝硬化的影响。方法肝硬化大鼠建模后随机分为4组:假手术组(sham)、假手术+ADSCs移植组(ADSCs)、脾切除组(Sp)和脾切除+ADSCs移植组(Sp+ADSCs)。假手术或脾切术后7 d经尾静脉注射ADSCs,7 d后检测肝功能、肝脏病理改变和ADSCs在肝内的归巢效率。结果分离的大鼠ADSCs CD29呈阳性且具备脂肪和软骨分化能力。ADSCs组肝纤维化面积较假手术组明显缩小(P<0.05)。与ADSCs组相比,Sp+ADSCs组的肝纤维化面积进一步缩小(P<0.05)。ADSCs组肝组织collagenⅠmRNA表达较sham组下调(P<0.05),且Sp+ADSCs组collagenⅠmRNA表达较ADSCs组进一步降低。Sp+ADSCs组ALT水平为(213±89)U/L,低于ADSCs组(280±56)U/L(P<0.05)。Sp+ADSCs组肝组织中荧光信号强度高于ADSCs组(P<0.05)。结论脾切除提高ADSCs在肝内的归巢效率并增强ADSCs对肝硬化的疗效。Objective To explore the influence of splenectomy on liver homing and treatment of cirrhosis after adipose tissue derived mesenchymal stem cells(ADSCs)infusion.Methods The rats liver cirrhosis model was established and randomly divided into four groups,sham operation group(sham),sham operation+ADSCs infusion group(ADSCs),splenectomy group(Sp),splenectomy+ADSCs infusion group(Sp+ADSCs).The cell infusion was performed one week after sham or splenectomy.Then,liver function,pathological and homing changes were detected one week later.Results The isolated ADSCs were CD29 positive and underwent adipogenic and chondrogenic differentiation,the hepatic fibrosis area in ADSCs group was significantly smaller than that sham operation group(P<0.05),and the fibrotic area in Sp+ADSCs group reduced as compared with that ADSCs group(P<0.05).The expression of collagenⅠmRNA in ADSCs group was significantly decreased as compared with that in sham group(P<0.05),and mRNA coding collagenⅠin Sp+ADSCs group was further decreased as comparedwith that in ADSCs group.As compared with ADSCs group[ALT:(280±56)U/L],Sp+ADSCs group[ALT:(213±89)U/L]had a significant difference in inhibiting liver injury(P<0.05);Ex vitro fluorescence imaging showed that the fluorescence signal intensity in liver tissue of the Sp+ADSCs was significantly higher than that in ADSCs group(P<0.05).Conclusions Splenectomy can significantly improve the live homing of ADSCs and improve their therapeutic effect on liver cirrhosis.

关 键 词：脾切除 脂肪间充质干细胞 归巢 肝硬化 

分 类 号：R575[医药卫生—消化系统]