In-vitro effect of Peganum harmala total alkaloids on spermatozoa quality and oxidative stress of epididymal ram semen  

作　　者：Hanane Derbak Mohamed Moussaoui Amine Benberkane Abdelhanine Ayad 

机构地区：[1]Department of Biological Sciences of the Environment,Faculty of Nature and Life Sciences,Universitéde Bejaia,06000 Bejaia,Algeria

出　　处：《Asian pacific Journal of Reproduction》2021年第5期232-238,共7页亚太生殖杂志（英文版）

摘　　要：Objective:To determine the in-vitro effect of the total alkaloid extract of Peganum(P.)harmala seeds on ram epididymal sperm.Methods:Semen was divided into six groups according to the following concentrations of the P.harmala total alkaloids:1,5,10,50,and 100μg/mL,and the control group.The samples were incubated at ambient temperature(21℃-24℃)for 24 h,and analyzed in terms of motility,membrane integrity,and oxidative status.Results:The sperm kinematic parameters,i.e.straight-line velocity,curvilinear velocity,average path velocity,were significantly higher when treated with P.harmala at concentrations ranging from 1 to 10μg/mL compared to the control group(P<0.05).In addtion,the highest amplitude of the lateral head displacement value was found in the groups treated with concentrations 1 and 5μg/mL of P.harmala compared to the control group(P<0.05).Total and progressive motilities showed that the extracts at 1,5,and 10μg/mL exhibited a high percentage after 24 h of incubation.The effect of P.harmala extracts on the membrane integrity of ram epididymal sperm was concentration-dependent and significantly different compared to the control group(P<0.05).Non-significantly lower lipid peroxidation levels were observed after 24 h of incubation of ram epididymal sperm treated with concentrations 1,5,and 10μg/mL of P.harmala extracts compared to the control group(P>0.05).Conclusions:Low concentrations(1-10μg/mL)of P.harmala extracts stimulate sperm motility,preserve membrane integrity and protect ram spermatozoa from lipid peroxidation.

关 键 词：ALKALOIDS Epididymal sperm MOTILITY Peganum harmala RAM SEMEN Membrane integrity Lipid peroxidation 1.Introduction 

分 类 号：R28[医药卫生—中药学]