不同类型饲粮纤维对葡聚糖硫酸钠诱导的结肠炎小鼠生长性能、血清生化指标和免疫相关指标的影响  

Effects of Different Types of Dietary Fibers on Growth Performance,Biochemical Indexes in Serum and Indexes Related to Immunity of Mice with Colitis Induced by Dextran Sulfate Sodium

作　　者：兰聪 何俊 李华 汤加勇 陈代文[1] 余冰[1] 何军[1] 黄志清[1] 郑萍[1] 毛湘冰[1] 虞洁[1] 罗钧秋[1] 阎辉 罗玉衡[1] LAN Cong;HE Jun;LI Hua;TANG Jiayong;CHEN Daiwen;YU Bing;HE Jun;HUANG Zhiqing;ZHENG Ping;MAO Xiangbing;YU Jie;LUO Junqiu;YAN Hui;LUO Yuheng(Sichuan Provincial Engineering Laboratory of Livestock and Poultry Nutrition&Feed,Animal Disease Resistance Nutrition Key Laboratory of Sichuan Province,Key Laboratory of Animal Disease Resistance Nutrition and Feed Science Ministry of Agriculture of China,Key Laboratory for Animal Disease Resistance Nutrition of China Ministry of Education,Animal Nutrition Institute of Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物营养研究所,动物抗病营养教育部重点实验室,农业部动物抗病营养与饲料重点实验室,动物抗病营养四川省重点实验室,四川省畜禽营养与饲料工程实验室,成都611130

出　　处：《动物营养学报》2021年第9期5277-5289,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31872369,31301987,32072743)。

摘　　要：本研究旨在比较分析菊粉(典型可溶性饲粮纤维)与微晶纤维素(MCC,典型不可溶性饲粮纤维)对葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠生长性能、血清生化指标和免疫相关指标的影响。选择8周龄的健康雄性C57BL/6小鼠24只,在其饮水中添加3%DSS(7 d)诱导其产生结肠炎后随机分为3组:C组(对照组)小鼠饲喂无纤维饲粮,I组和M组小鼠分别饲喂含有5%菊粉和MCC的饲粮,14 d后屠宰小鼠,收集静脉血和结肠样本。结果显示:饲粮中添加5%菊粉或MCC对DSS诱导的结肠炎小鼠恢复期生长性能以及肝脏、脾脏和胸腺指数均无显著影响(P>0.05)。与C组相比,I组和M组小鼠血清C反应蛋白含量显著降低(P<0.05),I组小鼠血清非酯化脂肪酸含量显著升高(P<0.05);与M组相比,I组小鼠血清非酯化脂肪酸含量显著升高(P<0.05),血清免疫球蛋白M含量有降低的趋势(P=0.061)。与C组相比,M组与I组小鼠脾脏中CD3+(P=0.053)和CD4+(P=0.091)T淋巴细胞的比例有升高的趋势。M组小鼠结肠组织中Toll样受体4(TLR4)、受体互作蛋白激酶2(RIPK2)的mRNA相对表达量显著低于C组(P<0.05);并且,M组小鼠结肠组织中TLR4、RIPK2、肿瘤坏死因子受体相关因子6(TRAF6)的mRNA相对表达量显著低于I组(P<0.05)。由此得出,5%菊粉或MCC均具有改善DSS诱导的结肠炎小鼠结肠免疫功能的作用,同时MCC能通过下调TLR4或核苷酸结合寡聚化结构域样受体(NLR)通路相关基因的表达来调控结肠免疫,提示不同类型饲粮纤维对结肠炎小鼠肠道健康的改善机理可能存在差异。This study was conducted to compare the effects of inulin(typical soluble dietary fiber)and microcrystalline cellulose(MCC,typical insoluble dietary fiber)on the growth performance,biochemical indexes in serum and indexes related to immunity of mice with colitis induced by dextran sulfate sodium(DSS).Twenty-four healthy male C57BL/6 mice aged 8 weeks were selected and 3%DSS was added to drinking water for 7 days to induce colitis.Then,mice with DSS-induced colitis were randomly allocated to three groups.Mice in C group(control group)were fed a fiber-free diet,while mice in I and M groups were fed diets containing 5%inulin or MCC for 14 days,respectively.At the end of the animal trial,all mice were sacrificed,and venous blood and colon samples were sampled.The results showed as follows:dietary supplementation of 5%inulin or MCC had no significant differences on the growth performance and the indexes of liver,spleen and thymus during recovery period(P>0.05).Compared with C group,the content of C-reactive protein in serum of mice in I and M groups was significantly decreased(P<0.05),while the content of non-esterified fatty acid in serum of mice in I group was significantly increased(P<0.05).Compared with M group,the content of non-esterified fatty acid in serum of mice in I group was significantly increased(P<0.05),and the content of immunoglobulin M in serum of mice tended to decrease(P=0.061).Meanwhile,compared with C group,the percentages of CD3+(P=0.053)and CD4+(P=0.091)T lymphocytes in spleen of mice in I and M groups had a tendency to increase.The mRNA relative expression levels of Toll like receptor 4(TLR4)and receptor-interacting serine/threonine protein kinase 2(RIPK2)in colon of mice in M group were significantly decreased compared with C group(P<0.05),and the mRNA relative expression levels of TLR4,tumor necrosis factor receptor associated factor 6(TRAF6)and RIPK2 in colon of these mice showed significantly decreased when compared with I group(P<0.05).Therefore,both the dietary supplementation of 5%inuli

关 键 词：小鼠 结肠炎 菊粉 微晶纤维素 肠道免疫 

分 类 号：S816[农业科学—饲料科学]