天麻素对阿尔茨海默病大鼠学习记忆能力和前额叶皮质GalR2表达的影响  

Effects of gastrodin on the learning and memory ability and GalR2 expression in prefrontal cortex of Alzheimer disease rats

作　　者：刘德玲 李怀斌[1] 黄锐[1] 赵健[1] LIU Deling;LI Huaibin;HUANG Rui;ZHAO Jian(Department of Human Anatomy,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院人体解剖学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2021年第5期426-429,共4页Journal of Wannan Medical College

基　　金：安徽省自然科学基金项目(1608085QH223);皖南医学院重点科研基金项目(WK2020Z12)。

摘　　要：目的:观察天麻素对阿尔茨海默病(AD)大鼠学习记忆能力和前额叶皮质甘丙肽受体2(GalR2)表达的影响,探讨天麻素对AD具有神经保护作用的可能机制。方法:40只雄性SD大鼠,随机分为正常组、模型组、天麻素+甘丙肽受体激动剂组、天麻素组、天麻素+甘丙肽受体抑制剂组,每组各8只。采用大鼠双侧海马注射Aβ1-42方法复制AD模型,用水迷宫检测大鼠行为学变化,Nissl染色观察大鼠前额叶皮质神经元的形态改变,免疫组织化学方法和Western blot法检测大鼠前额叶皮质GalR2的表达变化。结果:与正常组比较,模型组大鼠学习记忆能力下降(P<0.05),前额叶皮质神经元胞体皱缩,核仁不清,GalR2表达上调(P<0.05);与模型组比较,天麻素组大鼠GalR2的表达降低(P<0.05),学习记忆能力有所改善(P<0.05),天麻素+甘丙肽受体激动剂组GalR2的表达下调更为明显(P<0.05)。结论:天麻素可通过调节GalR2的表达来改善大鼠的学习记忆能力,具有一定的神经保护作用。Objective:To observe the effects of gastrodin on the learning and memory ability and the expression of galanin receptor 2(GalR2)in prefrontal cortex of model rats with Alzheimer disease(AD),and explore the possible mechanism of neuroprotective effect of gastrodin on AD.Methods:Forty male SD rats were randomly divided into normal group,model group,gastrodin+galanin receptor agonist group,gastrodin group,and gastrodin+galanin receptor inhibitor group(n=8 for each group).Rat models of AD were established by injecting Aβ1-42 into bilateral hippocampus,and observed for the changes of behaviors with water maze.The morphological changes of neurons in the prefrontal cortex were observed by Nissl staining,and GalR2 expression in the prefrontal cortex was detected by immunohistochemistry and Western blot.Results:The learning and memory ability of rats in model group was decreased(P<0.05),with shrunk cell bodies of neurons in prefrontal cortex,unclear nucleoli and significantly up-regulated GalR2 expression(P<0.05)as compared with normal group.Down-regulated GalR2 expression and improved learning and memory ability were observed in gastrodingroup,and down-regulation of GalR2 was more obvious in gostrodin+galanin receptor agonist group compared to model group(all P<0.05).Conclusion:Gastrodin can improve the learning and memory ability by regulating the expression of GalR2,and possesses certain neuroprotective effect in model rats of Alzheimer′s disease.

关 键 词：天麻素 阿尔茨海默病 前额叶皮质 甘丙肽受体 

分 类 号：R-332[医药卫生] R285.5