Negative Isolation of Circulating Tumor Cells Using a Microfluidic Platform Integrated with Streptavidin-Functionalized PLGA Nanofibers  

作　　者：Mengyuan Wang Yulong Tan Du Li Gangwei Xu Di Yin Yunchao Xiao Tiegang Xu Xiaofeng Chen Xiaoyue Zhu Xiangyang Shi 

机构地区：[1]State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,People’s Republic of China [2]Department of Thoracic Surgery,Huashan Hospital,Fudan University,Shanghai 200040,People’s Republic of China [3]State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,People’s Republic of China [4]Horticultural Plant Biology and Metabolomics Center,Fujian Agriculture and Forestry University,Fuzhou 350002,People’s Republic of China

出　　处：《Advanced Fiber Materials》2021年第3期192-202,共11页先进纤维材料（英文）

基　　金：the National Natural Science Foundation of China(21405012 and 81761148028);the Science and Technology Commission of Shanghai Municipality(19XD1400100 and 20DZ2254900);the Shanghai Education Commission through the Shanghai Leading Talents Program,and the 111 Project(BP0719035).

摘　　要：Detection of circulating tumor cells(CTCs)plays an important role in early diagnosis of cancer and personalized therapy.However,isolated CTCs,especially those captured by positive sorting methods,are difficult to culture in subsequent assays because the cells have to be labeled or attached to a substrate for separation.In this study,a negative sorting method has been developed for isolation of CTCs through a microfluidic platform integrated with streptavidin-functionalized electro-spun polylactic-co-glycolic acid nanofibers.Through the specific biotin-streptavidin interaction,the device is able to sort out biotinylated anti-CD45 antibody-labeled white blood cells(WBCs)and enrich A549 human cancer cells from the blood or CTCs from patient suffering non-small cell lung cancer.We demonstrate that the WBC capture efficiency is as high as 97.0%,and the recovery rate of cancer cells reaches up to 97.5%.CTCs are enumerated from blood samples of patients suf-fering lung carcinoma.The number of CTCs increased with the progression of NCCN TNM stages and showed statistically significant difference between stage I and later stages.These results suggest that the integrated negative sorting device is promising to be used for diagnosis of cancer.

关 键 词：Circulating tumor cells Electrospun nanofibers Microfluidic chips Negative sorting Non-small cell lung cancer 

分 类 号：R73[医药卫生—肿瘤]