Proximity labeling: an emerging tool for probing in planta molecular interactions  

作　　者：Xinxin Yang Zhiyan Wen Dingliang Zhang Zhen Li Dawei Li Ugrappa Nagalakshmi Savithramma P.Dinesh-Kumar Yongliang Zhang 

机构地区：[1]State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology,College of Biological Sciences,China Agricultural University,Beijing 100193,P.R.China [2]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China [3]Department of Plant Biology and the Genome Center,College of Biological Sciences,University of California,Davis,Davis,CA 95616,USA

出　　处：《Plant Communications》2021年第2期1-15,共15页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(31872637 to Y.Z.and 31830106 to D.L.);NSF-IOS-1354434;NSF-IOS-1339185;NIH-GM132582 to S.P.D.-K.

摘　　要：Protein–protein interaction(PPI)networks are key to nearly all aspects of cellular activity.Therefore,the identification of PPIs is important for understanding a specific biological process in an organism.Compared with conventional methods for probing PPIs,the recently described proximity labeling(PL)approach combined with mass spectrometry(MS)-based quantitative proteomics hasemerged as apowerful approach for characterizing PPIs.However,the application of PL in planta remains in its infancy.Here,we summarize recent progress in PL and its potential utilization in plant biology.We specifically summarize advances in PL,including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.

关 键 词：protein interactions proximity labeling biotin ligase plant membrane contact sites ORGANELLES 

分 类 号：Q94[生物学—植物学]