Erzhi pills ameliorates cognitive dysfunction and alter proteomic profiles of hippocampus in ovariectomized Alzheimer disease model rats induced by D-galactose and Aβ1-40 injection  

作　　者：XIE Yong-yan HOU Min ZHOU Mao-fu YAN Bo LIU Chao YAO Li-hua SUN Men-sheng HE Kun FANG Cong CHEN Yao-hui HUANG Li-ping LIU Bo 

机构地区：[1]School of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China [2]College of traditional Chinese medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China [3]the Second People′s Hospital of Jingdezhen,Jingdezhen 333099,China [4]Shandong Qidu Pharmaceutical Co.,Ltd.,Zibo 255400,China [5]Jiangxi Provincial People′s Hospital Affiliated to Nanchang University,Nanchang 330006,China [6]China Pharmaceutical University,Nanjing 211198,China [7]School of Life Science,Jiangxi Science&Technology Normal University,Nanchang 330096,China [8]不详

出　　处：《中国药理学与毒理学杂志》2021年第9期658-659,共2页Chinese Journal of Pharmacology and Toxicology

摘　　要：OBJECTIVE Erzhi pills is a clas⁃sic prescription of Chinese medicine originated from Fu Shou Jing Fang in Ming dynasty,with the effects of nourishing kidney-Yin and hemosta⁃sis,black hair,strengthening muscles and bones.As a classical prescription for nourishing kidney-Yin,Erzhi pills has been used to treat senile dementia in China for many years.Herein,our study aimed to investigate the protective effects of Erzhi pills in rat models of Alzheimer disease(AD)induced by ovariectomy as well as D-galactose and Aβ1-40 injection and to explore its potential mechanism.METHODS The model of AD rats was established by ovariectomy com⁃bined with D-galactose and Aβ1-40 injection.Ovariectomized rats were randomly divided into four groups:model group,estradiol valerate(0.80 mg·kg-1)group,Erzhi pills high(1.50 mg·kg-1)and low(0.75 mg·kg-1)doses group.In addition,rats of sham operation were selected as the sham operated group.Except for the sham oper⁃ated group,rats were injected intraperitoneally with D-galactose(100 mg·kg-1 per day,for 49 d)on the 8th day,then they were given intracerebro⁃ventricular injection of Aβ1-40(10μg per rat,1 g·L-1)on the 36th day,while the corresponding drugs were given by gastrointestinal administra⁃tion on the 22nd day.In our study,Morris water maze test was used to evaluate the learning and memory abilities,while ELISA kit was used to an⁃alyze serum estrogen level.The morphology of hippocampal neuron cells was observed by HE staining,and Nissl staining was utilized to ob⁃serve the Nissl body in cytoplasm.Then,the ex⁃pression of ERβpositive cells and hippocampal Aβ1-40 and p-Tau404 proteins was determined by immunohistochemistry.In order to further explore the molecular mechanism of Erzhi pills preven⁃tion and treatment of AD,proteomics was used to find potential targets and related pathways,Western blotting was used to verify the expres⁃sion of candidate differential protein.According to the results of proteomics in our experiment,Western blotting was used to dete

关 键 词：Erzhi pills Alzheimer disease hip⁃pocampal neurons proteomics ESTROGEN aging memory PI3K/Akt signaling pathway 

分 类 号：R285.5[医药卫生—中药学]