Baroreflex impairment exacerbates LPS-induced inflammation in rat cerebral cortex and hippocampus  

作　　者：JI Wei QI Hui-bin ZHU De-rong FENG Zhao-yang KONG De-ping ZHANG Fang-fang YU Hai-yang GAO Yong-feng TAN Rui ZHAO Xiao-min 

机构地区：[1]Institute of Pharmacology,Shandong First Medical University&Shandong Academy of Medical Sciences,Tai′an 271016,China

出　　处：《中国药理学与毒理学杂志》2021年第9期679-679,共1页Chinese Journal of Pharmacology and Toxicology

基　　金：Natural Science Foundation of Shandong Province(ZR2017MH048)。

摘　　要：OBJECTIVE Platelets play a major role in mediating inflammatory response.The present work was designed to investigate whether arterial baroreflex impairment induced by sinoaortic denervation(SAD)affect platelet activation,leading to the exacerbation of cerebral cortex and hippocampus inflammation in rats.METHODS Adult male SD rats were divided into four groups:the sham control,the sinoaortic denervation(SAD),the sham+LPS,the SAD+LPS.In another experiment,there were also four groups:the sham control,the SAD,the SAD+LPS and the SAD+LPS+asprin.Four weeks after sham or SAD surgery,all rats were examined for the level of CD41,CD45,IL-1βand PF-4 in the cerebral cortex and hippocampus using immunofluorescence and ELISA.Blood platelet and leukocyte count,platelet microaggre⁃gation,expression of CD154 and CD62P on platelet surface and platelet-leukocyte aggregate level was detected by flow cytometry.RESULTS Compared with sham+LPS group,the in SAD+LPS group rats exhibited the high level of CD41,CD45,IL-1βand PF-4 in the cerebral cortex and hippocampus.Leukocyte count,platelet microag⁃gregation,expression of CD154 and CD62P on platelet surface and platelet-leukocyte aggregate level was increased,while blood platelet count was decreased in the SAD+LPS.Moreover,all the above changes were improved in the SAD+LPS+asprin group when compared with the SAD+LPS group.CONCLUSION Arterial baroreflex dysfunction exacerbates inflammation in the rat cerebral cortex and hippocampus,which is likely mediated by platelet.

关 键 词：arterial baroreflex INFLAMMATION PLATELET ASPIRIN 

分 类 号：R338[医药卫生—人体生理学]