Discovery of alkaloids from the leaves of Isatis indigotica Fortune with neuroprotective activity  

作　　者：XI Yu-Fei LOU Li-Li HAN Feng-Ying WANG Xiao-Bo HUANG Xiao-Xiao YAO Guo-Dong SONG Shao-Jiang 

机构地区：[1]Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research&Development,Liaoning Province,School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China [2]Chinese People's Liberation Army Logistics Support Force No.967 Hospital,Dalian 116000,China

出　　处：《Chinese Journal of Natural Medicines》2021年第9期680-685,共6页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82003635 and 81973462);the Liaoning Revitalization Talents Program(No.XLYC2007180);the Program for Major Scientific and Medical Technology Problems of China Medicine Education Association in 2020(No.2020KTS003);the Natural Science Foundation of Liaoning Province of China(No.2019-MS-303);the Scientific Research Fund Project of Liaoning Provincial Department of Education(No.2019LJC05)。

摘　　要：Seven alkaloids including five undescribed ones(1 a/1 b,2,3 and 5)were obtained from the leaves of Isatis indigotica Fortune.Their structures were established by extensive spectroscopic analyses.The absolute configurations of compounds 1 a,1 b,3 and 5 were determined by comparison of the experimental and calculated electronic circular dichroism(ECD)spectra.Subsequently,the neuroprotective effects of all the isolates against H_(2)O_(2)-induced injury in SH-SY5 Y cells were evaluated in vitro by MTT assay.Moreover,Annexin V-FITC/PI double staining was performed,while the activities of antioxidant enzymes(SOD,CAT and GSH-Px)for compounds 1 a and 1 b were measured.

关 键 词：Isatis indigotica ALKALOIDS Neuroprotective effects Structure elucidation 

分 类 号：R284[医药卫生—中药学]