Sesquiterpenes and polyphenols with glucose-uptake stimulatory and antioxidant activities from the medicinal mushroom Sanghuangporus sanghuang  

作　　者：ZHANG Jin-Jin CHEN Bao-Song DAI Huan-Qin REN Jin-Wei ZHOU Li-Wei WU Sheng-Hua LIU Hong-Wei 

机构地区：[1]State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]Savaid Medical School,University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Biology,National Museum of Natural Science,Taichung 40453,Taiwan,China

出　　处：《Chinese Journal of Natural Medicines》2021年第9期693-699,共7页中国天然药物（英文版）

基　　金：supported by the National Key R&D Program of China(No.2018YFD0400203);the National Natural Science Foundation of China(No.81673334)。

摘　　要：A chemical investigation on the fermentation products of Sanghuangporus sanghuang led to the isolation and identification of fourteen secondary metabolites(1-14)including eight sesquiterpenoids(1-8)and six polyphenols(9-14).Compounds1-3 were sesquiterpenes with new structures which were elucidated based on NMR spectroscopy,high resolution mass spectrometry(HRMS)and electronic circular dichroism(ECD)data.All the isolates were tested for their stimulation effects on glucose uptake in insulin-resistant HepG2 cells,and cellular antioxidant activity.Compounds 9-12 were subjected to molecular docking experiment to primarily evaluate their anti-coronavirus(SARS-CoV-2)activity.As a result,compounds 9-12 were found to increase the glucose uptake of insulin-resistant HepG2 cells by 18.1%,62.7%,33.7%and 21.4%at the dose of 50μmol·L^(-1),respectively.Compounds 9-12 also showed good cellular antioxidant activities with CAA_(50)values of 12.23,23.11,5.31 and 16.04μmol·L^(-1),respectively.Molecular docking between COVID-19 M^(pro)and compounds 9-12 indicated potential SARS-CoV-2 inhibitory activity of these four compounds.This work provides new insights for the potential role of the medicinal mushroom S.sanghuang as drugs and functional foods.

关 键 词：Sanghuangporus sanghuang SESQUITERPENOIDS POLYPHENOLS Glucose-uptake stimulation Cellular antioxidant activity Anti-coronavirus activity 

分 类 号：R284[医药卫生—中药学] R965[医药卫生—中医学]