基于DNA-Ag/Pt双金属纳米簇比色法检测葡萄糖  

Colorimetric Detection of Glucose Based on DNA-Templated Ag/Pt Bimetallic Nanoclusters

作　　者：傅贤明 许丽兰 陈健敏 FU Xianming;XU Lilang;CHEN Jianmin(School of Pharmacy and Medical Technology,Putian University;Key Laboratory of Pharmaceutical Analysis and Laboratory Medicine Putian University,Putian,Fujian 351100,China)

机构地区：[1]莆田学院药学与医学技术学院 [2]药物分析与检验医学福建省高校重点实验室,福建莆田351100

出　　处：《九江学院学报（自然科学版）》2021年第3期90-95,共6页Journal of Jiujiang University：Natural Science Edition

基　　金：莆田市科技计划项目(编号2018SP3004);福建省中青年教师教育科研项目(编号JAT170512)的成果之一。

摘　　要：葡萄糖是血糖含量的指标。在人体血糖含量上升的过程中,通常伴随着人体某些疾病的产生,比如高血压、高血脂、眼疾等。因此,快速、简便、灵敏的定量检测葡萄糖对临床分析与诊断具有重要意义。研究发现,以DNA为模板合成的银铂双金属纳米簇具有过氧化物模拟酶活性,其催化能力强弱在一定范围内依赖于H 2 O 2的浓度。基于此特性,文章构建了一种比色传感器用于葡萄糖的检测。实验结果表明,在最佳条件下,该比色传感器对葡萄糖的检测范围为400~900μmol/L,检测限为200μmol/L,且传感器对葡萄糖的检测具有高选择性,并可应用于实际血清样品中葡萄糖的检测。该比色传感器成本低、操作简便、灵敏度高、选择性好,具有潜在的应用价值。Glucose was an indicator of blood sugar content.In the process of human blood sugar rising,it was usually accompanied by some diseases,such as hypertension,hyperlipidemia,eye diseases and so on.Therefore,the development of a rapid,simple and sensitive method for quantitative detection of glucose was of great significance for clinical analysis and diagnosis.It was found that the Ag/Pt bimetallic nanoclusters synthesized by DNA template possessed intrinsic peroxidase-like activity and its catalytic activity was H 2 O 2 concentration dependent in a certain range.Based on this characteristic,a colorimetric sensor was constructed for glucose detection.The experimental results showed that under the optimal conditions,the detection range of glucose was 400~900μmol/L and the detection limit was 200μmol/L.The sensor had high selectivity for glucose detection,and could be applied to the detection of glucose in actual serum samples.The sensor had the advantages of low cost,simple operation,high sensitivity and good selectivity,which had potential application value.

关 键 词：葡萄糖 银铂 双金属纳米簇 模拟酶 比色传感器 

分 类 号：R-33[医药卫生]