PDLLA/β-TCP/HA/CHS/NGF Sustained-release Conduits for Peripheral Nerve Regeneration  

作　　者：YAN Xiumei WANG Jing HE Qundi XU Haixing TAO Junyan KORAL Kelly LI Kebi XU Jingyi WEN Jing HUANG Zhijun XU Peihu 闫秀美;WANG Jing;HE Qundi;徐海星;TAO Junyan;KORAL Kelly;LI Kebi;XU Jingyi;WEN Jing;HUANG Zhijun;许沛虎(Department of Pharmaceutical Engineering,School of Chemistry,Chemical Engineering and Life Science,Wuhan University of Technology,Wuhan 430070,China;China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd.,Shenzhen 518029,China;Wuhan Mafangshan Middle School,Wuhan 430070,China;Department of Pathology,University of Pittsburgh.Pittsburgh,PA,USA)

机构地区：[1]Department of Pharmaceutical Engineering,School of Chemistry,Chemical Engineering and Life Science,Wuhan University of Technology,Wuhan 430070,China [2]China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd.,Shenzhen 518029,China [3]Wuhan Mafangshan Middle School,Wuhan 430070,China [4]Department of Pathology,University of Pittsburgh.Pittsburgh,PA,USA

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2021年第4期600-606,共7页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the Chinese National Natural Science Foundation (No. 51572206);the National Innovation and Entrepreneurship Training Program for College (Nos. 202110497032, 202110497033, and S202110497080)。

摘　　要：Using nerve guide conduits (NGCs) to promote the regeneration of PNI is a feasible alternative to autograft.Compared with NGCs made of single material,composite NGCs have a greater development prospect.Our previous research has confirmed that poly(D,L-lactic acid)/β-tricalcium phosphate/hyaluronic acid/chitosan/nerve growth factor (PDLLA/β-TCP/HA/CHS/NGF) NGCs have excellent physical and chemical properties,which can slowly release NGF and support cell adhesion and proliferation.In this study,PDLLA/β-TCP/HA/CHS/NGF NGCs were prepared and used to bridge a 10 mm sciatic nerve defect in 200-250 g Sprague-Dawley (SD) rat to verify the performance of the NGCs in vivo.Substantial improvements in nerve regeneration were observed after using the PDLLA/β-TCP/HA/CHS/NGF NGCs based on gross post-operation observation,triceps wet weight analysis and nerve histological assessment.In vivo studies illustrate that the PDLLA/β-TCP/HA/CHS/NGF sustained-release NGCs can effectively promote peripheral nerve regeneration,and the effect is similar to that of autograft.

关 键 词：SUSTAINED-RELEASE composite nerve conduits peripheral nerve regeneration 

分 类 号：TB332[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]