微小RNA-92a在非小细胞肺癌中的表达及其生物学行为特性实验研究  

Experimental study on the expression of microRNA-92a in non-small cell lung cancer and its biological behavior characteristics

作　　者：李志强[1] 张和平[1] 陈凯[1] Li Zhiqiang;Zhang Heping;Chen Kai(Department of Respiratory and Critical Care Medicine,Baoji Central Hospital,Baoji 721008,China)

机构地区：[1]宝鸡市中心医院呼吸与危重症医学科,721008

出　　处：《国际呼吸杂志》2021年第17期1327-1332,共6页International Journal of Respiration

基　　金：陕西省卫生健康科研基金(2018SF-218)。

摘　　要：目的探究微小RNA-92a(miR-92a)在非小细胞肺癌(NSCLC)组织中的表达及对其细胞生物学行为的影响。方法实验性研究。收集宝鸡市中心医院2018年1月至2020年10月收治的39例NSCLC患者癌组织和癌旁正常组织,采用RT-PCR检测组织中miR-92a表达水平,并分析其与临床病理的关系。另选用肺癌细胞株A549,将miR-92a mimics、miR-92a inhibitor及对应阴性对照质粒转染A549细胞,转染48 h后采用MTT法检测细胞增殖水平,采用划痕实验检测细胞迁移能力。结果NSCLC组织中miR-92a相对表达量(2.58±0.47)高于正常组织(1.04±0.39),差异有统计学意义(P<0.05);TNM分期T3+T4期、中低分化、淋巴结转移的NSCLC患者癌组织中miR-92a mRNA相对表达量分别(2.68±0.35)、(2.84±0.26)、(3.25±0.68)高于T1+T2期、高分化、淋巴结未转移者(2.19±0.30)、(2.13±0.38)、(2.17±0.54),差异有统计学意义(P<0.05);体外实验结果显示,miR-92a mimics组细胞中miR-92a mRNA相对表达量、增殖水平、迁移能力高于mimics-NC组,miR-92a inhibitor组细胞miR-92a mRNA相对表达量、增殖水平、迁移能力低于inhibitor-NC组,差异有统计学意义(P<0.05)。结论miR-92a在NSCLC组织中表达上调,且与患者临床病理有关,下调miR-92a表达可抑制NSCLC细胞的增殖、转移能力。Objective To explore the expression of microRNA-92a(miR-92a)in non-small cell lung cancer(NSCLC)tissues and its influence on cell biological behavior.Methods This was an experimental research.Thirty-nine cancer tissues and adjacent normal tissues from NSCLC patients between January 2018 and October 2020 in Baoji Central Hospital were selected.RT-PCR was used to detect the expression level of miR-92a in the tissues and its relationship with clinicopathology was analyzed.Another lung cancer cell line A549 was also chosen.MiR-92a mimics,miR-92a inhibitor and the corresponding negative control plasmids were transfected into A549 cells.After transfection 48 h,the cell proliferation level was measured by MTT method,and the cell migration ability was detected by scratch test.Results The relative expression of miR-92a in NSCLC tissues 2.58±0.47 was higher than that of normal tissues 1.04±0.39(P<0.05).The relative expression of miR-92a mRNA in cancer tissues of NSCLC patients with T3+T4 stage,moderately poorly differentiated,and lymph node metastasis 2.68±0.35,2.84±0.26,3.25±0.68 was higher than that in T1+T2 stage,well-differentiated,and non-metastasis patients 2.19±0.30,2.13±0.38,2.17±0.54(P<0.05).In vitro experiment showed that miR-92a mRNA expression,proliferation levels and migration ability were higher in miR-92a mimics group than in mimics-NC group;the miR-92a mRNA expression,proliferation level and migration ability were lower in miR-92a inhibitor group than in inhibitor-NC group(P<0.05).Conclusions The expression of miR-92a is up-regulated in NSCLC tissues and is related to the clinical pathology of patients.Down-regulating the expression of miR-92a can inhibit the proliferation and metastasis of NSCLC cells.

关 键 词：癌 非小细胞肺 miR-92a 生物学行为 侵袭转移 

分 类 号：R734.2[医药卫生—肿瘤]