细胞水平染料木素协同维生素D_(3)的抗骨质疏松作用  

Synergistic Anti-osteoporosis Effect of Genistein and Vitamin D_(3) in the Cellular Level

作　　者：赵佳[1] 姜波[1] 郭建丽 张丹洋 孙文辉 包永明[1,3] Zhao Jia;Jiang Bo;Guo Jianli;Zhang Danyang;Sun Wenhui;Bao Yongming(School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning;Panjin Institute of Industrial Technology,Dalian University of Technology,Panjin 124221,Liaoning;School of Ocean Science and Technology,Dalian University of Technology,Panjin 124221,Liaoning)

机构地区：[1]大连理工大学生物工程学院,辽宁大连116024 [2]大连理工大学盘锦产业技术研究院,辽宁盘锦124221 [3]大连理工大学海洋科学与技术学院,辽宁盘锦124221

出　　处：《中国食品学报》2021年第9期19-29,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金面上项目(21878041)。

摘　　要：维生素D_(3)(Vitamin D_(3),VD_(3))和染料木素(Genistein,Gen)二者均具有抗骨质疏松作用,然而,二者协同作用机制及维生素D_(3)的抗骨质疏松作用使用剂量有待阐明。本研究报告了维生素D_(3)和染料木素对人成骨肉瘤细胞MG-63、小鼠巨噬细胞RAW264.7诱导的破骨细胞增殖与分化的影响机制。维生素D_(3)联合染料木素可显著促进成骨细胞的增殖分化,促进骨保护素(OPG)的表达,抑制核因子κB(NF-κB)受体激活剂(RANKL)的表达,提高OPG和RANKL mRNA表达比值。此外,维生素D_(3)在6.25~50.00μmol/L剂量范围,可促进破骨细胞的增殖和成熟,而联合染料木素后表现为显著抑制作用。维生素D_(3)可促进破骨细胞中组织蛋白酶K的表达,联合染料木素处理细胞,显著抑制酒石酸抗性磷酸酶(TRAP)和组织蛋白酶K的表达。试验结果表明,维生素D_(3)联合染料木素,可显著促进成骨细胞的增殖和分化。维生素D_(3)表现为浓度依赖的增强破骨细胞增殖和成熟作用,而联合染料木素可显著抑制破骨细胞增殖和成熟,间接起到抗骨质疏松作用。Vitamin D_(3) is a fat-soluble vitamin synthesized endogenously in the skin,and genistein is an isoflavone mainly found in legumes.Both have anti-osteoporosis effects,and the two compounds combination also promotes bone formation.However,the synergistic mechanism of the two and the dosage of anti-osteoporosis effect of vitamin D_(3) need to be clarified.In this study,the effect of vitamin D_(3) and genistein on the proliferation and differentiation of osteoclasts induced by human osteosarcoma cell MG-63 and mouse macrophage RAW264.7 was reported.The osteoblast(MG-63)proliferation was increased significantly by VD_(3) and genestein combination,and osteoblast indicators of alkaline phosphatase(ALP)activity,calcium nodules,the expression of osteoprotegerin(OPG),ratio of OPG and receptor activator of nuclear factor kappa B(NF-κB)ligand(RANKL)in mRNA level were also promoted significantly in combination group,compared with both single groups.In addition,the proliferation and maturation of osteoclasts(RAW264.7 RANKL induced)were promoted by VD_(3) with 6.25-50.00μmol/L dosage,but inhibited significantly by combination of VD_(3) and genistein.Furthermore,the expression of cathepsinK mRNA in osteoclasts was promoted by VD_(3),but the expression of tartrate resistant phosphatase(TRAP)and CathepsinK mRNA were inhibited in the combination group.These results showed that the proliferation and differentiation of osteoblasts could be significantly promoted by vitamin D_(3) combined with genistein,indicating bone density increase directly.Vitamin D_(3),showed a dose dependent promoting effect on osteoclast proliferation and maturation,could inhibit osteoclast proliferation and maturation significantly,playing an anti-osteoporosis role indirect after combined with genistein.

关 键 词：维生素D_(3) 染料木素 成骨细胞 破骨细胞 骨质疏松症 

分 类 号：R580[医药卫生—内分泌]