Coproduction of xylose and biobutanol from corn stover via recycling of sulfuric acid pretreatment solution  

作　　者：Jin-Jun Dong Bao-Jian Ma Yong-Mei Liu Hao Li Lei Gong Rui-Zhi Han Guo-Chao Xu Ye Ni 

机构地区：[1]Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China [2]School of Pharmacy,Taizhou Polytechnic College,Taizhou 225300,Jiangsu,China [3]Key Laboratory of Guangxi Biorefinery,Nanning 530003,Guangxi,China

出　　处：《Systems Microbiology and Biomanufacturing》2021年第2期200-207,共8页系统微生物学与生物制造（英文）

基　　金：This work was supported by National Natural Science Foundation of China(31601463);National Key R&D Program of China(2018YFA0901700);National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-07);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX19_1834);Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.

摘　　要：Sulfuric acid was used in the pretreatment of corn stover to obtain xylose as a value-added by-product,and the pretreated corn stover(Pre-CS)was hydrolyzed to produce glucose for butanol fermentation.The aim of this work is to achieve high xylose accumulation and reduced wastewater by recycling the pretreatment solution.The pretreatment conditions were optimized as follows:180°C,15 min,1:7 solid-liquid ratio(w/w),0.6%H_(2)SO_(4)(w/w,first batch)/0.9%H_(2)SO_(4)(w/w,second and third batches),in which pretreatment solution was recycled for three times.Under above conditions,pretreatment solution containing 56.3 g/L xylose and 4.5 g/L glucose was obtained.Pre-CS residue was further hydrolyzed by cellulase to achieve 35.7-39.9 g/L glucose.The condensed corn stover hydrolysate was subjected to simultaneous detoxification and sterilization using 1%(w/w)activated carbon and then applied in butanol fermentation.The highest butanol titer of 9.5 g/L was obtained in 72 h.The results provide a practical approach for coproducing xylose and biobutanol from corn stover.

关 键 词：Acid pretreatment RECYCLE XYLOSE BUTANOL Simultaneous detoxification and sterilization Clostridium saccharobutylicum 

分 类 号：X70[环境科学与工程—环境工程]