败酱草对对乙酰氨基酚诱导的大鼠药物性肝损伤的保护作用  

Herba Patriniae protects against paracetamol-induced liver injury in rats

作　　者：唐文诚 何清 宋大萍[2] 沈政洪 王继生 TANG Wencheng;HE Qing;SONG Daping;SHEN Zhenghong;WANG Jisheng(School of pharmacy,Southwest Medical University,Luzhou 646000,China;Mianyang Third People's Hospital,Sichuan mental health center,Mianyang 621000)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]绵阳市第三人民医院•四川省精神卫生中心,四川绵阳621000

出　　处：《中国比较医学杂志》2021年第9期78-83,共6页Chinese Journal of Comparative Medicine

基　　金：绵阳市科技局课题(2019YFZJ018)。

摘　　要：目的败酱草是败酱科多年生草本植物黄花败酱、白花败酱的带根全草,具有多种药理活性。本文旨在探究败酱草对对乙酰氨基酚诱导的大鼠药物性肝损伤的保护作用。方法采用尾静脉注射对乙酰氨基酚将大鼠随机分为6组:对照组、模型组、败酱草低剂量组、败酱草中剂量组、败酱草高剂量组、联苯双脂组。造模同时败酱草低、中、高剂量组每日灌胃败酱草4、8、16 g/kg,联苯双脂组每日灌胃联苯双脂0.3 g/kg,对照组和模型组灌胃等量生理盐水,连续30 d。计算肝脏指数;HE染色检测肝组织病理损伤程度;试剂盒检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)水平;酶联免疫吸附实验检测白介素6(IL-6)、肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)水平;通过试剂盒检测肝组织匀浆中总超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量。结果与对照组相比较,模型组肝脏指数显著升高(P<0.05),AST、ALT、ALP水平显著升高(P<0.05),TNF-α、IL-6、IL-1β水平显著升高(P<0.05),SOD、GSH-Px含量显著降低(P<0.05)、MDA含量显著升高(P<0.05)。与模型组相比较,败酱草中、高剂量组和联苯双酯组肝脏指数显著降低(P<0.05),病理损伤程度明显改善,AST、ALT、ALP水平显著降低(P<0.05),TNF-α、IL-6、IL-1β水平显著降低(P<0.05),SOD、GSH-Px含量显著升高(P<0.05)、MDA含量显著降低(P<0.05)。结论败酱草通过改善肝组织病理损伤、抑制炎症因子的释放、改善氧化应激对对乙酰氨基酚诱导的大鼠药物性肝损伤的具有保护作用。Objective Herba Patriniae is a root grass of perennial herbaceous plants in the family Caprifoliaceae,with various pharmacological activities. This study was conducted to investigate the protective effect of Herba Patriniae on paracetamol-induced liver injury in rats. Methods Rats were intravenously injected with acetaminophen and then randomly divided into the control,model,low-dose Herba Patriniae,medium-dose Herba Patriniae,high-dose Herba Patriniae and bifendatatum groups. The low-,medium-and high-dose Herba Patriniae groups were gavaged with 4,8 and 16 g/kg Herba Patriniae once per day,respectively. The bifendatatum group received 0. 3 g/kg bifendatatum,and the control and model groups were given the same amount of normal saline for 30 consecutive days. The liver index was calculated. Hematoxylin and eosin staining was used to detect the degree of pathological injury to the liver tissue. Serum ALT,AST and ALP levels were detected using kits. Tumor necrosis factor-α( TNF-α),interleukin-6( IL-6) and interleukin-1β( IL-1β) were determined via enzyme-linked immunosorbent assay. Superoxide dismutase( SOD),MDA and GSH-Px contents in the liver homogenate were detected using kits. Results Compared with those of the control group,the liver index of the model group was significantly increased,the AST,ALT,ALP,TNF-α,IL-6 and IL-1β levels were significantly increased,the SOD and GSH-Px contents were significantly reduced, and the MDA content was significantly increased( all P<0. 05).Compared with those of the model group,the liver indexes of the Herba Patriniae and bifendatatum groups were significantly reduced,the degree of pathological damage was significantly attenuated,the AST,ALT,ALP,TNF-α,IL-6 and IL-1βlevels were significantly decreased,the SOD and GSH-Px contents were significantly increased,and the MDA content was significantly decreased( all P<0. 05). Conclusions Herba Patriniae protected rats from paracetamol-induced liver injury by alleviating pathological injury to the liver tissue,inhibiting the rel

关 键 词：药物性肝损伤 败酱草 对乙酰氨基酚 氧化应激 

分 类 号：R-33[医药卫生]