TGR、LGR5联合RRM1检测在非小细胞肺癌疾病进展中的评估作用  

Evaluation of TGR and LGR5 combined with RRM1 detection in the progression of non-small cell lung cancer

作　　者：李琪[1] 王继灵[2] 曹荣娟 刘秀珍[1] 孟祥云[1] LI Qi;WANG Jiling;CAO Rongjuan;LIU Xiuzhen;MENG Xiangyun(Department of Pharmacy,Hefei Second People's Hospital,Hefei,Anhui,China,230011;Department of Respiration,Hefei Second People's Hospital,Hefei,Anhui,China,230011;Department of Pharmacy,Hefei People's Hospital,Hefei,Anhui,China,230011)

机构地区：[1]合肥市第二人民医院药学部,安徽合肥230011 [2]合肥市第二人民医院呼吸科,安徽合肥230011 [3]合肥市人民医院药学部,安徽合肥230011

出　　处：《分子诊断与治疗杂志》2021年第9期1524-1528,共5页Journal of Molecular Diagnostics and Therapy

基　　金：安徽省卫生计生委科研计划项目(12070403012)。

摘　　要：目的分析肿瘤生长速率(TGR)、富含亮氨酸的G蛋白偶联受体5(LGR5)和核苷酸还原酶M1(RRM1)在非小细胞肺癌(NSCLC)中的表达及疾病进展中的评估作用。方法选取2015年1月至2018年1月于合肥市第二人民医院就诊的97例NSCLC患者,取所有患者术中新鲜肺癌组织(NSCLC组)及距离癌组织5 cm处癌旁组织(对照组),比较不同组织TGR值及LGR5、RRM1的表达情况。分析影响NSCLC患者预后生存的相关因素及TGR、LGR5、RRM1对患者预后生存的影响。结果NSCLC组LGR5阴性率及RRM1阳性率高于对照组,差异均有统计学意义(P<0.05)。中低分化、Ⅲ+Ⅳ期、有淋巴结侵犯者TGR升高率、LGR5阴性率及RRM1阳性率高于高分化、Ⅰ+Ⅱ期及无淋巴结侵犯者,差异有统计学意义(P<0.05)。中低分化、Ⅲ+Ⅳ期、有淋巴结侵犯、TGR升高、LGR5阴性及RRM1阳性表达为影响NSCLC患者预后生存的独立危险因素(P<0.05)。TGR、LGR5、RRM1单项及联合检测AUC分别为0.820、0.715、0.821、0.905,各指标AUC以联合检测最大(P<0.05)。TGR降低、LGR5阳性组及RRM1阴性组平均生存时间长于TGR升高、LGR5阴性组、RRM1阳性组,差异均有统计学意义(P<0.05)。结论 TGR、LGR5、RRM1与NSCLC疾病进展、预后均有着密切关系,可作为预测患者预后生存的分子标志物和肿瘤治疗的潜在靶点。Objective To analyze the expression of tumor growth rate(TGR),Leucine-rich repeat-containing G protein coupled receptor 5(LGR5) and ribonucleotide reductase subunit M1(RRM1) in the evaluation of disease progression of non-small-cell lung cancer(NSCLC).Method 97 NSCLC patients treated in this hospital from January 2015 to January 2018 were selected.Fresh lung cancer tissues(NSCLC group)and adjacent tissue 5 cm from the cancerous tissues(control group) were taken from all patients during the surgery.The TGR value and the expression of LGR5 and RRM1 in different tissues were compared.The relevant factors that affected the prognostic survival of NSCLC patients and the effects of TGR,LGR5,and RRM1 on the prognostic survival of patients were analyzed.Result The negative rate of LGR5 and the positive rate of RRM1 in the NSCLC group were higher than those in the control group,and the differences were statistically significant(P<0.05).The rate of TGR elevated,LGR5 negative rate,and RRM1 positive rate in poorly differentiated,stage Ⅲ+Ⅳ,and lymph node invasion was higher than that in well-differentiated,stage Ⅰ+Ⅱ and no lymph node invasion,the difference was statistically significant(P<0.05).Poorly differentiated,stage Ⅲ+Ⅳ,lymph node invasion,elevated TGR,negative LGR5 and positive expression of RRM1 were independent risk factors affecting the prognosis of NSCLC patients(P<0.05).The AUC of TGR,LGR5,RRM1 single detection and combined detection were 0.820,0.715,0.821,0.905,respectively,and the AUC of the combined detection was the largest(P<0.05).The average survival time of TGR decreased,LGR5 positive group and RRM1 negative group was longer than TGR increased,LGR5 negative group,RRM1 positive group,the difference was statistically significant(P<0.05).Conclusion TGR,LGR5,and RRM1 are closely related to NSCLC disease progression and prognosis,and can be used as molecular markers for predicting patient survival and potential targets for tumor therapy.

关 键 词：TGR LGR5 RRM1 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]