A novel technology to reduce astringency of tea polyphenols extract and its mechanism  

作　　者：Jin-yan Wan Yu Long Yu-lu Zhang Yan Xiang Song-yu Liu Nan Li Ding-kun Zhang 

机构地区：[1]College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

出　　处：《Chinese Herbal Medicines》2021年第3期421-429,共9页中草药（英文版）

基　　金：supported by the National Natural Science Foundation of China.(Nos.81673615,81403115)。

摘　　要：Objective:Tea polyphenols are natural extracts used widely throughout the world.However,the severe astringency of tea polyphenols has reduced patient compliance.Based on the analysis of the formation mechanism of astringency,this paper hopes to propose a new method to control the astringency of tea polyphenols and improve patient compliance without changing its effect.Methods:Artificial saliva was used to prepare the tea polyphenols solution with different p H,using bcasein to imitate salivary protein,and preparing 1.2 mg/m L b-casein solution.A fluorescence quenching test was used to study the interaction between tea polyphenols and b-casein,combined with the stability test results of the compound,we can choose the p H with weak binding but good stability as the best p H for masking astringency.The taste-masking tablets were prepared under the best p H conditions,and the Xinnaojian Original Tablets were prepared according to the conventional preparation method.The disintegration time limit and solubility were tested respectively.The astringency of Xinnaojian original tablets and taste-masking tablets was evaluated by visual analogue scale(VAS).Results:The result of the fluorescence quenching test prompted that the combination force was the weakest when the p H was 4.9.Further synchronous fluorescence analysis showed that an increase in p H resulted in a decrease of the binding sites between tea polyphenols and b-casein,and this decrease was closely related to changes in tryptophan residues in b-casein.Both original and taste-masking Xinnaojian Tablets were prepared.Volunteers’VAS scores illustrated that the astringency improved significantly with the masking tablets(P<0.05).Conclusion:This p H-adjusting masking treatment had little effect on the recovery of polyphenols from the tablets or the dissolution of the tablets.This study provides a novel and feasible astringency masking technology for tea polyphenols and its preparation.

关 键 词：ASTRINGENCY b-casein fluorescence quenching tea polyphenols 

分 类 号：R283.6[医药卫生—中药学]