S100钙结合蛋白A6在鉴别良恶性胸腔积液中的价值  

Value of S100 calcium-binding protein A6 in the differentiation of benign and malignant pleural effusion

作　　者：屈晓一 王婷 白洋 QU Xiaoyi;WANG Ting;BAI Yang(Xi′an People′s Hospital·Xi′an Fourth Hospital:Department of Respiratory Medicine,Xi′an 710004,China;Xi′an People′s Hospital·Xi′an Fourth Hospital:Department of Ultrasonography,Xi′an 710004,China)

机构地区：[1]西安市人民医院·西安市第四医院呼吸内科,陕西西安710004 [2]西安市人民医院·西安市第四医院超声科,陕西西安710004

出　　处：《西北国防医学杂志》2021年第7期662-666,共5页Medical Journal of National Defending Forces in Northwest China

基　　金：陕西省重点研发计划资助项目(2018SF-219);西安市科技计划项目(201805093YX1SF27)。

摘　　要：目的:通过比较S100钙结合蛋白A6(S100 calcium-binding protein A6,S100A6)在不同类型胸腔积液中的表达差异,评估这一标记物鉴别良恶性胸腔积液的价值。方法:收集我院2017-1-1~2020-12-31住院的104例非小细胞肺癌患者及96例结核性渗出性胸膜炎患者的胸腔积液,使用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)测定S100A6的表达水平,比较S100A6在两组中的表达差异,绘制受试者操作特性(receiver operating characteristic,ROC)曲线图。同时,测定S100A6在两组患者血清中的表达水平,与胸水表达值作比较。另外,从住院病历中采集信息,记录常用肺癌标记物,包括癌胚抗原(carcinoembryonic antigen,CEA),细胞角蛋白可溶性片段211(cytokeratin soluble fragment 211,CYFRA211)和神经元特异性烯醇化酶(neurogen specific enolase,NSE),在两组中的表达情况。结果:和其他经典肺癌标记物类似,对比结核性胸腔积液患者,S100A6在非小细胞肺癌患者的胸水及血清中的表达均明显升高,且在胸水中升高更为明显(P<0.01)。ROC曲线显示,S100A6可区分良恶性胸腔积液,曲线下面积(area under curve,AUC)=0.797,其在胸腔积液的诊断价值高于血清(AUC=0.894)。结论:S100A6在恶性胸腔积液中表达升高,未来有望作为区分良恶性胸腔积液的标记物运用于临床。Objective:To compare the expression of S100 calcium binding protein A6(S100A6)in different types of pleural effusion,and to evaluate the value of S100A6 in differentiating benign and malignant pleural effusion.Methods:The pleural effusions of 104 patients with non-small cell lung cancer and 96 patients with tuberculous exudative pleurisy in our hospital from January 2017 to December 2020 were collected.The expression level of S100A6 was determined by enzyme linked immunosorbent assay(ELISA),Receiver operating characteristic(ROC)curve was drawn.At the same time,the expression level of S100A6 in serum of the two groups were measured,and compared with the expression value of pleural effusion.In addition,information was collected from inpatient records to record the expression of common lung cancer markers,including carcinoembryonic antigen(CEA),cytokeratin soluble fragment 211(cyfra211)and neuron specific enolase(NSE).Results:Similar to other classic lung cancer markers,the expression of S100A6 in pleural effusion and serum of patients with tuberculous pleural effusion was significantly higher than that of patients with tuberculous pleural effusion(P<0.01).ROC curve showed that S100A6 could distinguish benign and malignant pleural effusion,area under curve(AUC=0.797),and its diagnostic value in pleural effusion was higher than that in serum(AUC=0.894).Conclusion:The expression of S100A6 is increased in malignant pleural effusion,which may be used as a marker to distinguish benign and malignant pleural effusion.

关 键 词：非小细胞肺癌 标记物 胸腔积液 S100A6 

分 类 号：R734.2[医药卫生—肿瘤]