牡丹花瓣药材的定性鉴别与含量测定  

Qualitative identification and content determination of Paeonia suffruticosa Andr.petals

作　　者：肖会敏 杨旭 罗欢欢 李雨欣 李捷 林奋 罗定强 王四旺 XIAO Huimin;YANG Xu;LUO Huanhuan;LI Yuxin;LI Jie;LIN Fen;LUO Dingqiang;WANG Siwang(Research and Development Department of Shaanxi Fengdan Zhengyuan Biotechnology Limited Company,Xi’an 710077,China;Department of Life Science and Medicine,Northwest University;Department of Traditional Chinese Medicine and Natural Medicine,Air Force Medical University;Shaanxi Institute of Food and Drug Supervision and Inspection)

机构地区：[1]陕西凤丹正元生物科技有限公司研发部,西安710077 [2]西北大学生命科学与医学部 [3]空军军医大学中药与天然药物教研室 [4]陕西省食品药品监督检验研究院

出　　处：《山西医科大学学报》2021年第9期1210-1216,共7页Journal of Shanxi Medical University

基　　金：陕西省生物医药重点实验室后补助项目(2018SZS41)。

摘　　要：目的建立牡丹(Paeonia suffruticosa Andr.)花瓣药材质量标准的研究方法。方法采用生物显微镜对牡丹花瓣药材粉末进行显微鉴别;采用薄层色谱法(TLC)鉴别牡丹花瓣药材中的芍药苷;依据2020年版《中国药典》四部,对11批牡丹花瓣药材,采用烘干法测定水分的含量,采用直接灰分法测定总灰分与酸不溶性灰分的含量,采用冷浸法测定水溶性浸出物的含量;采用高效液相色谱法(HPLC)测定11批牡丹花瓣药材中没食子酸与芍药苷的含量。结果花瓣粉末显微鉴别发现薄壁细胞、导管、花粉粒、螺纹导管。牡丹花瓣药材中芍药苷的TLC鉴别方法专属性强、重复性良好。11批药材中水分、总灰分、酸不溶性灰分和水溶性浸出物的质量分数分别为6.95%-8.11%,9.87%-12.31%,0.26%-0.45%和36.08%-41.98%。没食子酸在9.75-624.25μg/ml浓度范围内线性关系良好(r=0.9991),平均加样回收率为98.58%,RSD为1.10%;芍药苷在6.90-441.72μg/ml浓度范围内线性关系良好(r=0.9992),平均加样回收率为98.70%,RSD为1.31%。11批药材中没食子酸和芍药苷的质量分数分别为0.194%-0.267%和0.629%-0.755%。结论通过对牡丹花瓣药材HPLC含量测定、TLC鉴别及显微鉴别的研究,为牡丹花瓣药材质量标准的建立提供了依据。Objective To establish the methods for the quality standard of Paeonia suffruticosa Andr.petals.Methods Biological microscope was used to identify Paeonia suffruticosa Andr.petal powders.TLC was used to identify paeoniflorin in the Paeonia suffruticosa Andr.petals.According to the fourth part of Chinese Pharmacopoeia(2020 Edition),the water content of 11 batches of medicinal materials was determined by drying method,the total ash content and the acid insoluble ash content were determined by direct ash method,and the water-soluble extract content was determined by cold leaching method.The contents of gallic acid and paeoniflorin in 11 batches of Paeonia suffruticosa Andr.petals were determined by HPLC.Results Parenchyma cells,ducts,pollen grains and threaded ducts were observed in petal powder.TLC identification method of paeoniflorin in traditional Chinese medicine with strong specificity and good repeatability was established.The mass fractions of water,total ash,acid insoluble ash and water-soluble extract in 11 batches of Paeonia suffruticosa Andr.petals were 6.95%-8.11%,9.87%-12.31%,0.26%-0.45%,36.08%-41.98%,respectively.The linear relationship of gallic acid was good in the range of 9.75-624.25μg/ml(r=0.9991),and its average reco-very of sample was 98.58%and RSD was 1.10%.And the linear relationship of paeoniflorin was 6.90-441.72μg/ml(r=0.9992),and its average recovery rate of samples was 98.70%and RSD was 1.31%.The mass fractions of gallic acid and paeoniflorin in 11 batches of Paeonia suffruticosa Andr.petals were 0.194%-0.267%and 0.629%-0.755%,respectively.Conclusion The study provides the basis for the establishment of the quality standard of Paeonia suffruticosa Andr.petals.

关 键 词：牡丹 薄层色谱法 高效液相色谱法 显微鉴别法 芍药苷 没食子酸 

分 类 号：R927.2[医药卫生—药学]