牙周炎患者外周血miRNA表达谱分析评价  

Differential Expression Profiles of miRNAs in Blood of Periodontitis Patients

作　　者：张正 郑友丽[3] 边晓为[3] ZHANG Zheng;ZHENG Youli;BIAN Xiaowei(Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China;Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin 300041, China;Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China.)

机构地区：[1]天津市口腔医院,南开大学医学院,天津300041 [2]天津市口腔功能重建重点实验室,天津300041 [3]天津医科大学口腔医院,天津300070

出　　处：《口腔医学研究》2021年第10期894-899,共6页Journal of Oral Science Research

基　　金：天津市自然科学基金(编号:20JCQNJC00200);天津市卫生健康委员会科技项目(编号:KJ20041)。

摘　　要：目的:分析牙周炎患者外周血微小RNA(microRNA,miRNA)表达谱,筛选出具有潜在牙周炎诊断价值的外周血miRNA,为牙周炎特异性诊断方式的探寻提供参考。方法:从基因表达综合数据库(Gene Expression Omnibus,GEO)获取受试者外周血miRNA表达谱数据集(GSE61741),并进行GEO2R在线工具分析。Logistic回归分析进行差异表达miRNA自变量筛选,依据接受者操作特征(receiver operating characteristic,ROC)曲线下面积(area under the curve,AUC)确定miRNA的诊断价值。结果:与健康对照组相比,牙周炎患者外周血123个miRNA表达水平显著下调,109个miRNA表达水平显著上调;Logistic回归分析筛选出3个与牙周炎发病相关的外周血miRNA,即hsa-miR-361-5p、hsa-miR-663和hsa-miR-744;ROC曲线显示,hsa-miR-361-5p、hsa-miR-663、hsa-miR-744以及三者联合诊断牙周炎的AUC值分别为0.871、0.899、0.925和0.981,均具有统计学意义(P<0.01)。结论:牙周炎患者外周血miRNA表达谱与健康对照组存在明显差异,hsa-miR-361-5p、hsa-miR-744和hsa-miR-663与牙周炎发病风险相关,对于牙周炎的诊疗具有一定价值。Objective:To characterize the microRNA(miRNA)profiles in blood of periodontitis patients.Methods:One miRNA expression profile(GSE61741)was downloaded from the GEO database and analyzed using GEO2R.Logistic regression analysis was used to screen independent variables among differentially expressed miRNAs(DE-miRNAs).The diagnostic values of screened miRNAs were determined by the area under the receiver operating characteristic(ROC)curve(AUC).Results:A total of 232 DE-miRNAs,consisting of 109 upregulated DE-miRNAs and 123 downregulated DE-miRNA,were screened.Logistic regression analysis showed that there were three miRNAs related to periodontitis,namely hsa-mir-361-5p,hsa-mir-663,and hsa-mir-744.The AUC values of hsa-mir-361-5p,hsa-mir-663,hsa-mir-744,and their combination in the diagnosis of periodontitis were 0.871,0.899,0.925,and 0.981,respectively(P<0.01).Conclusion:MiRNAs are expressed differentially in periodontitis patients.hsa-mir-361-5p,hsa-mir-744,and hsa-mir-663 were associated with the risk of periodontitis and had potential value in the diagnosis of periodontitis.

关 键 词：牙周炎 微小RNA 发病风险 诊断 

分 类 号：R73[医药卫生—肿瘤]