柱前在线衍生-高效液相色谱法同时测定复方丹参胶囊中19种氨基酸  

Simultaneous determination of 19 amino acids in Composite Salvia Miltiorrhiza Capsules by high performance liquid chromatography with online pre-column derivatization

作　　者：许有诚 赵庄 李丹凤 Xu Youcheng;Zhao Zhuang;Li Danfeng(Guangxi Institute For Food and Drug Control,Nanning 530021,China)

机构地区：[1]广西壮族自治区食品药品检验所,南宁530021

出　　处：《化学分析计量》2021年第10期1-6,共6页Chemical Analysis And Meterage

基　　金：广西科技计划(重点研发计划)项目(桂科AB18221125)。

摘　　要：建立柱前在线衍生-高效液相色谱法同时测定复方丹参胶囊中19种氨基酸含量的方法。采用Agilent HPH-C18(4.6 mm×150 mm,2.7μm)色谱柱,以10 mmol/L硼酸钠-磷酸氢二钠缓冲液(用6 mol/L盐酸调节pH值至8.2)为流动相A,乙腈-甲醇-水(体积比为41∶41∶18)溶液为流动相B,梯度洗脱,流量为1.0 mL/min,柱温为40℃,检测波长分别为338 nm和262 nm,进样体积为1μL。19种氨基酸在各自的质量浓度范围内与色谱峰面积呈良好的线性关系,相关系数为0.9994~0.9999,方法检出限为0.06~0.68μg/mL。样品加标回收率为91.4%~106.5%,测定结果的相对标准偏差为0.1%~1.8%(n=6)。该方法可用于复方丹参胶囊中多种氨基酸的同时测定。A method of the simultaneous determination of 19 amino acids contents in Composite Salvia Miltiorrhiza Capsules by high performance liquid chromatography with online pre-column derivatization was established.The chromatographic column was Agilent HPH-C18(4.6 mm×150 mm,2.7μm).The mobile phase A was 10 mmol/L sodium borate-disodium hydrogen phosphate buffer(adjusted to pH 8.2 with 6 mol/L hydrochloric acid),and the mobile phase B was acetonitrile-methanol-water(the volume ratio was 41∶41∶18)with gradient elution.The flow rate was 1.0 mL/min,the column temperature was 40℃,the detection wavelength was 338 nm and 262 nm,and the injection volume was 1μL.The mass concentration of 19 amino acids had a good linear relationship with the chromatographic peak area in their respective range,the correlation coefficients were 0.9994-0.9999,and the method detection limits were 0.06-0.68μg/mL.The average recoveries were 91.4%-106.5%,and the relative standard deviations of determination results were 0.1%-1.8%(n=6).The method can be used for the simultaneous determination of multiple amino acids in Composite Salvia Miltiorrhiza Capsules.

关 键 词：复方丹参胶囊 氨基酸 柱前衍生 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]