Wnt/β&catenin信号通路参与马蹄内翻足畸形形成的研究  

Wnt/β-catenin signaling pathway involved in the deformity of congenital talipes equinovarus

作　　者：李汾杰 陈曦 李洋杰 廖佳馨 李志富 刘桉茁 张月秋 颜南[2] 王正东[3] Li Fenjie;Chen Xi;Li Yangjie;Liao Jiaxin;Li Zhifu;Liu Anzhuo;Zhang Yueqiu;Yan Nan;WangZhengdong(Basic Medical Colleg,,Shenyang Medical College,Shenyang 110034,China;Department of Rehabilitation,Shenyang Medical College,Shenyang 110034,China;Department of Anatomy,Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院基础医学院,沈阳110034 [2]沈阳医学院康复教研室,沈阳110034 [3]沈阳医学院解剖学教研室,沈阳110034

出　　处：《中国临床解剖学杂志》2021年第5期575-578,585,共5页Chinese Journal of Clinical Anatomy

基　　金：辽宁省大学生创新创业训练计划(S202010164003)。

摘　　要：目的探讨Sox9(sex determining region Y-box9)和β-catenin对先天性马蹄内翻足(congenital talipes equinovarus,CTE)的影响以及Wnt/β-catenin信号通路作用机制。方法将孕10 d的SD大鼠随机均分为实验组及对照组,以135 mg/kg全反式维甲酸溶于矿物油对实验组大鼠进行灌胃制作胎鼠CTE模型,对照组予以等量矿物油灌胃处理,取大鼠足踝部组织,通过免疫组化、RT-PCR、Western blot检测β-catenin、Sox9以及磷酸化β-catenin-S552的表达水平。结果与对照组相比,HE染色可见CTE模型组织中有较多的胶原组织沉积,免疫组化结果显示实验组标本Sox9与β-catenin表达增高,RTqPCR表明实验组Sox9与β-catenin的mRNA水平显著增高,Western blot结果显示实验组Sox9与β-catenin的表达增高而磷酸化β-catenin-S552表达降低。结论CTE大鼠的足踝部组织中Sox9高表达受Wnt/β-catenin信号通路调控,该通路参与先天性马蹄内翻足畸形的形成。Objective To investigate the effect of sex determining region Y-box 9(Sox9),β-catenin and Wnt/β-catenin signaling pathway on the congenital talipes equinovarus(CTE)and its mechanism.Methods SD rats at 10 days of gestation were randomly divided into an experimental group and a control group.All trans retinoic acid(135 mg/kg)was dissolved in mineral oil to make fetal rat CTE model in the experimental group.The control group was given the same amount of mineral oil.Immunohistochemistry,RTPCR and Western blot were used to detect the expression levels ofβ-catenin,Sox9 and phosphorylatedβ-catenin-s552 in ankle soft tissue of control group and model group.Results Compared with the control group,HE staining showed more collagen deposition in CE model tissues,immunohistochemical results showed that the expression ofβ-catenin and Sox9 in the model group significantly increased.RT-qPCR result showed that the mRNA level of Sox9,β-catenin increased.Western blot result showed that the expression of P-β-catenin-s552 increased,the expression of Sox9 andβ-catenin in the experimental group decreased.Conclusions The high expression of Sox9 in the ankle tissue of rats with congenital clubfoot is regulated by Wnt/β-catenin signaling pathway,which is involved in the formation of congenital clubfoot.

关 键 词：先天性马蹄内翻足 SOX9 Wnt信号通路 Β-CATENIN P-β-catenin 

分 类 号：R363.2[医药卫生—病理学]