林芝桦褐孔菌中化学成分的研究  

Chemical Constituents from Inonotus obliquus Distributed in Nyingchi

作　　者：刘怡 刘振东 汪雯翰[2] LIU Yi;LIU Zhendong;WANG Wenhan(Food Science College,Tibet Agriculture&Animal Husbandry Universit,Nyingchi Tibet,860000;INational Engineering Research Center of Edible Fungi,Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture,Shanghai Key Laboratory Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]西藏农牧学院食品科学学院,西藏林芝860000 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验,上海201403

出　　处：《高原农业》2021年第5期497-503,共7页Journal of Plateau Agriculture

基　　金：西藏自治区科技计划项目(重大专项)(XZ201801-GA-15),项目名称:林芝桦褐孔菌活性化合物抑制非小细胞肺癌的分子机制研究。

摘　　要：研究纤孔菌属的桦褐孔菌化学成分。运用硅胶柱色谱、MCI gel树脂、LH-20葡聚糖凝胶(Sephadex LH-20)柱色谱、HPLC、ODS等色谱分离手段,对桦褐孔菌的甲醇提取物的乙酸乙酯部位进行分离纯化,通过其氢谱和碳谱数据与文献进行对照,鉴定其结构。共分离了9个化合物,分别鉴定为:5,7,22-三烯-3β-羟基-麦角甾(HH-1)、(22E)-5a,8a-epidioxyergosta-6,22-dien-3b-ol(HH-2)、桦褐孔菌萜C(HH-3)、羊毛甾醇(HH-4)、inonotsutriol A(HH-5)、Inonotusane B(HH-6)、Euphol(HH-7)、3β-乙酰齐墩果酸(HH-8)、巨豆-5-烯-3,9-二醇(HH-9)。To investigate the chemical constituents of Inonotus obliquus distributed in Nyingchi,the chemical constituents were isolated and purified by chromatography with silica gel,Sephadex LH-20,and their structures were identified by analysis of spectroscopic evidence and physicochemical properties as well as relevant references.A total of 9 compounds were isolated from plant material extracted with aqueous methanol,which were elucidated as ergosta-5,7,22-triene-3β-ol(HH-1),(22E)-5a,8a-epidioxyergosta-6,22-dien-3b-ol(HH-2),inoterpene C(HH-3),lanosterol(HH-4),inonotsutriol A(HH-5),inonotusane B(HH-6),Euphol(HH-7),3β-acetoxyurs-12-ene-28-oic acid(HH-8),Megastigm-5-ene-3,9-diol(HH-9).

关 键 词：桦褐孔菌 桦褐孔菌醇 乌索酸 羊毛甾醇 

分 类 号：R284.1[医药卫生—中药学]