基于16S rDNA技术研究黄连解毒汤对db/db糖尿病小鼠肠道菌群的影响  

Effects of Huanglian Jiedu Decoction on gut microbiota in db/db mice based on 16S rDNA sequencing technology

作　　者：何文娇 胡甜 石晶晶 曹东敏 潮金鸣 陈超[1,2,3] 陈云波 汤丹[1,2,3] 王淑美 HE Wen-jiao;HU Tian;SHI Jing-jing;CAO Dong-min;CHAO Jin-ming;CHEN Chao;CHEN Yun-bo;TANG Dan;WANG Shu-mei(Guangdong Pharmaceutical University,Guangzhou 510006,China;Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of National Administration of Traditinal Chinese Medicine,Guangzhou 510006,China;Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Province,Guangzhou 510006,China;Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广东药科大学,广州510006 [2]国家中医药管理局中药数字化质量评价技术重点研究室,广州510006 [3]广东省中药质量工程技术研究中心,广州510006 [4]广州中医药大学科技创新中心,广州510405

出　　处：《中华中医药杂志》2021年第8期5024-5028,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82074137,No.81773884,No.81674040);广东省科技计划项目(No.2017A020213029);广东省中医药局科研项目(No.20181066);广东省自然科学基金项目(No.2020A1515011515)。

摘　　要：目的:研究黄连解毒汤对db/db小鼠肠道菌群的影响。方法:将db/m小鼠作为空白组,db/db小鼠随机分为模型组、黄连解毒汤组和二甲双胍组;给药12周后,全自动生化仪检测血浆中血糖血脂指标[血糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)],使用16S rDNA技术检测各组小鼠粪便肠道菌群变化及预测db/db小鼠肠道菌群相关的功能通路。结果:与模型组比较,黄连解毒汤组体质量、TG和LDL-C水平降低(P<0.05);黄连解毒汤组肠道菌群从门到属水平均发生了变化,有益菌的丰度增加,有害菌的丰度降低,其中,Parabacteroides_goldsteinii、多形拟杆菌、纤线杆菌目、革兰菌属和鼠乳杆菌显著增多;共发现35条功能通路与db/db小鼠肠道菌群相关,其中碳水化合物代谢、能量代谢和糖合成与代谢等通路与糖尿病相关。结论:黄连解毒汤具有改善db/db糖尿病小鼠肠道菌群失调的作用。Objective:To investigate the effects of Huanglian Jiedu Decoction on gut microbiota modulation in db/db mice.Methods:The db/m mice were used as the control group and db/db mice were randomly divided into model group,Huanglian Jiedu Decoction group and Metformin group.After intragastric administration for twelve weeks,the blood glucose and lipid index were analyzed by an automatic biochemical analyzer,the changes of intestinal microflora were determined and functional pathways related to intestinal flora in db/db mice were predicted by 16S rDNA sequencing technology.Results:Compared with the model group,Huanglian Jiedu Decoction could effectively reduce the level of weight,triglyceride(TG)and low density lipoprotein-cholesterol(LDL-C)(P<0.05);the intestinal flora of Huanglian Jiedu Decoction group had changed from phylum units to the genus units,with increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria,among them,the abundance of Parabacteroides_goldsteinii,bacteroides thetaiotaomicron,ktedonobacterales,gramella and lactobacillus murinus were significantly increased.A total of 35 functional pathways were found to be related to the intestinal flora of db/db mice,of which carbohydrate metabolism,energy metabolism,glycan biosynthesis and metabolism pathways were related to diabetes.Conclusion:Huanglian Jiedu Decoction has the effect of improving the intestinal microflora imbalance in db/db mice.

关 键 词：黄连解毒汤 糖尿病 肠道菌群 16S rDNA技术 DB/DB小鼠 功能通路 

分 类 号：R285.5[医药卫生—中药学]