富氢水缓解5-FU诱导的大鼠口腔粘膜溃疡  

Alleviation effects of drinking hydrogen-rich water on the oral mucosal ulcer induced by 5-fluorouracil in rats

作　　者：张单雅 薛俊莉 刘伯言 陶鸽如 石运芝 秦树存 Zhang Danya;Xue Junli;Liu Boyan;Tao Geru;Shi Yunzhi;Qin Shucun(School of Stomatology,Shandong First Medical University&Shandong Academy of Medical Sciences,Taian 271000,China;Life Sciences Research Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Taian 271000,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)口腔医学院,山东泰安271000 [2]山东第一医科大学(山东省医学科学院)生命科学研究中心,山东泰安271000

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第9期662-666,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：山东省大学生创新创业训练计划(20191828)。

摘　　要：目的评估饮用富氢水对化疗药物5-氟尿嘧啶(5-FU)诱导口腔粘膜溃疡的缓解效果和可能的生物学作用。方法雄性SD大鼠连续2日腹腔注射5-FU(0.05 mg/g),第3日应用NaOH颗粒或20%乙酸接触颊部粘膜建立口腔溃疡模型,第5日给予实验大鼠饮用富氢水,于第10日处死动物取材。统计实验组大鼠存活率,观察口腔粘膜损伤情况,并使用Image J软件测量溃疡面积,ELISA法检测大鼠血清中TNF-α、IL-6、IL-1β的水平,免疫组织化学法检测颊粘膜中TNF-α、IL-6和iNOS的表达。结果饮用富氢水可提高实验组大鼠存活率;NaOH颗粒制造的溃疡,损伤面积大于注射20%乙酸造成的溃疡损伤,但饮用富氢水对溃疡损伤面积的影响差异无统计学意义(P>0.05)。模型组大鼠血清中TNF-α、IL-6、IL-1β的含量均高于对照组,富氢水组较模型组有下降趋势但仅20%乙酸模型组IL-1β与对照组比较差异有统计学意义,余差异均无统计学意义。颊粘膜中TNF-α和IL-6在健康对照组中的表达较弱,在模型组中的阳性着色见于颊粘膜上皮细胞和炎性间质中;富氢水组的阳性着色较模型组减弱;iNOS在实验组颊粘膜中的阳性表达较少。结论氢分子可能通过抑制氧化应激相关炎症反应,减轻5-FU诱导的大鼠口腔粘膜溃疡。Objective:To investigate the alleviation effects of drinking hydrogen-rich water on the oral mucosal ulcer induced by 5-fluorouracil(5-FU)and its possible biological mechanism.Methods:Male SD rats were intraperitoneally injected with 5-FU(0.05 mg/g)for 2 consecutive days.On D3,rats were treated with NaOH particles or 20%acetic acid on buccal mucosa to establish oral ulcer model.From D5,the rats were given hydrogen-rich water,and the animals were sacrificed on the D10.The survival rates of rats in all experimental groups were counted,and the lesions of oral mucosa were measured by Image J software.The levels of TNF-α,IL-6 and IL-1βin serum were detected by ELISA,and the expressions of TNF-α,IL-6 and iNOS at buccal mucosa were detected by immunohistochemistry.Results:Drinking hydrogen-rich water improved the survival rate of rats in the experimental groups.The damage area of ulcers produced by NaOH particles were larger than that caused by injection with 20%acetic acid(P<0.05),but there was no significant difference in the damage area of ulcers with drinking hydrogen-rich water or not(P>0.05).The serum levels of TNF-α,IL-6 and IL-1βof rats in model groups were significantly higher than those in the control group,while levels of the hydrogen-rich water group showed a downward trend compared with the model group.The expression of TNF-αand IL-6 at buccal mucosa were low in the healthy control and high in the model groups,while the expression in hydrogen-rich water groups showed a downward trend compared with the model group.The expression of iNOS at buccal mucosa was low in all groups.Conclusion:Molecular hydrogen alleviates oral mucosal ulcers induced by 5-FU in rats possibly through inhibiting oxidative stress-related inflammation.

关 键 词：富氢水 5-FU 口腔粘膜溃疡 大鼠 

分 类 号：R780[医药卫生—口腔医学]