牙髓血运重建对大鼠年轻恒牙影像及VEGF、NGF的影响  

Effects of Pulp Revascularization on the Image of Young Permanent Teeth and VEGF,NGF in Rats

作　　者：刘迪[1] 史滨伟[1] 陶冠男 LIU Di;SHI Binwei;TAO Guannan(Department of Stomatology,Nangang Hospital,Heilongjiang Provincial Hospital,Harbin Heilongjiang 150080,China)

机构地区：[1]黑龙江省医院南岗院区口腔科,黑龙江哈尔滨150080

出　　处：《中国卫生标准管理》2021年第18期113-116,共4页China Health Standard Management

基　　金：黑龙江省卫生健康委科研课题(名称:牙髓血运重建术对年轻恒牙根部组织VEGF、NGF表达的影响,编号:2019-123)。

摘　　要：目的探讨牙髓血运重建术在大鼠年轻恒牙中的作用及对血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)、神经生长因子(nerve growth factor,NGF)的影响。方法大鼠20只中的随机10只大鼠为对照组;剩余大鼠建立大鼠年轻恒牙牙髓血运重建术的模型,设为观察组。采用实时荧光PCR法测定VEGF、NGF mRNA水平,分析牙髓血运重建术对年轻恒牙大鼠VEGF、NGF的影响。结果X线结果表明:观察组牙髓血运重建组大鼠下颌第一磨牙牙根尖部阴影膨大,管壁增厚,有新生高密度硬组织形成,牙根继续发育。实时荧光PCR法结果表明:观察组大鼠牙髓血运重建术干预后VEGF、NGF mRNA水平高于对照组(P<0.05)。结论牙髓血运重建术用于年轻恒牙大鼠中效果理想,能提高VEGF、NGF水平。Objective To investigate the effects of pulp revascularization on vascular endothelial growth factor(VEGF)and nerve growth factor(NGF)in young permanent teeth rats.Methods 20 rats were randomly divided into control group(n=10)and observation group(n=10).The mRNA levels of VEGF and NGF were measured by real-time PCR,and the effects of pulp revascularization on VEGF and NGF in young permanent teeth rats were analyzed.Results The X-ray results showed that the root tip shadow of the mandibular first molar in the pulp revascularization group was enlarged,the canal wall was thickened,new high-density hard tissue was formed,and the root continued to develop in the observation group.The results of real-time PCR showed that the mRNA levels of VEGF and NGF in the observation group were higher than those in the control group(P<0.05).Conclusion Endodontic revascularization in young permanent teeth rats has ideal effect and can improve the levels of VEGF and NGF.

关 键 词：牙髓血运重建术 年轻恒牙 血管内皮细胞生长因子 神经生长因子 实时荧光PCR法 下颌第一磨牙 

分 类 号：R781[医药卫生—口腔医学]