普萘洛尔对胶质母细胞裸鼠移植瘤模型的干预作用  

THE INTERVENTION EFFECT OF PROPRANOLOL ON XENOGRAFT NUDE MOUSE MODEL FOR GLIOBLASTOMA

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作  者:刘世霞 刘艳[2] 周良健 李兴兰 赵琪[2] LIU Shi-xia;LIU Yan;ZHOU Liang-jian;LI Xing-lan;ZHAO Qi(Jinzhou Medical University,Jinzhou 121001;Linyi Peopl's Hospital,Linyi 276000,China)

机构地区:[1]锦州医科大学,辽宁锦州121001 [2]临沂市人民医院,山东临沂276000

出  处:《山东医学高等专科学校学报》2021年第5期339-341,共3页Journal of Shandong Medical College

基  金:山东省医药卫生科技发展计划项目(No.2017WS324)。

摘  要:目的探讨普萘洛尔对胶质母细胞瘤的干预效果。方法应用U251细胞系建立裸鼠移植瘤模型,待平均瘤体增长至约100 mm 3体积时,将移植瘤模型裸鼠随机分为对照组和实验组,对照组给予安慰剂、实验组给予普萘洛尔灌胃,观察裸鼠体重及肿瘤生长情况,第13天处死小鼠,采集肿瘤组织行VEGFA、HIF1A免疫组化检测。结果两组肿瘤体积及VEGFA蛋白表达无统计学意义(P>0.05),HIF1A蛋白表达有统计学意义(χ^(2)=4.70,P=0.03)。结论普萘洛尔可抑制U251细胞系裸鼠移植瘤模型肿瘤的HIF1A蛋白表达,对肿瘤体积及VEGFA蛋白表达无明显影响。Objective To investigate the intervention effect of propranolol on glioblastoma.Methods U251 cell line was used to establish a nude mouse xenograft tumor model.When the average tumor size increased to about 100 mm 3,the xenograft nude mice were randomly divided into a control group and an experimental group.The control group was given a placebo and the experimental group was given propranolol.Two groups were administered intragastrically to observe the body weight and tumor growth of nude mice.The mice were sacrificed on the 13th day,and tumor tissues were collected for immunohistochemical detection of VEGFA and HIF1A.Results The tumor volume and VEGFA protein expression in the two groups were not statistically significant(P>0.05),and the HIF1A protein expression was statistically significant(χ^(2)=4.70,P=0.03).Conclusion Propranolol can inhibit the expression of HIF1A protein in the U251 cell line transplanted tumor model tumor in nude mice,and has no significant effect on the tumor volume and the expression of VEGFA protein.

关 键 词:胶质母细胞瘤 裸鼠移植瘤模型 普洛萘尔 

分 类 号:R739.41[医药卫生—肿瘤]

 

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