番泻苷B对胃癌细胞生长、凋亡、干样特性以及IL-6/JAK2/STAT3通路的影响  

Effects of sennoside B on the growth,apoptosis,stem-like properties and IL-6/JAK2/STAT3 pathway of gastric cancer cells

作　　者：李毅 李文忠 师路 杜泽东 高志勇 王聆宇 LI Yi;LI Wenzhong;SHI Lu;DU Zedong;GAO Zhiyong;WANG Lingyu(Department of Gastrointestinal Surgery,Chengdu 363 Hospital,Southwest Medical University,Chengdu 610097,China;Department of Anesthesiology,Chengdu 363 Hospital,Southwest Medical University,Chengdu 610097,China)

机构地区：[1]西南医科大学附属成都三六三医院胃肠外科,成都610097 [2]西南医科大学附属成都三六三医院麻醉科,成都610097

出　　处：《免疫学杂志》2021年第10期861-867,共7页Immunological Journal

基　　金：四川省卫生和计划生育委科研课题(18PJ195)。

摘　　要：目的探讨番泻苷B对胃癌细胞生长、凋亡、干样特性以及IL-6/JAK2/STAT3通路的影响。方法通过CCK8法检测胃癌细胞SGC-7901的活力,筛选合适剂量;流式检测细胞凋亡;显微观察干细胞成球;流式分选干细胞CD133+;Western blot检测干细胞标志物(OCT4、SOX2);ELISA检测炎症因子(iNOS、IL-6、IL-4)含量;Western blot检测IL-6/JAK2/STAT3通路相关蛋白(IL-6、p-JAK2、p-STAT3)表达。结果CCK8检测显示,随番泻苷B浓度的增加,对细胞生长的抑制作用显著(P<0.05),筛选4个剂量组(0、5、10、20μmol·L-1)进行后续实验。与0μmol·L-1剂量组相比,5μmol·L-1剂量组差异不具有显著性,10、20μmol·L-1剂量组细胞凋亡率、抗炎因子IL-4含量显著增加(P<0.05),干细胞成球数量及直径、CD133+比例、干细胞标志物(OCT4、SOX2)表达、促炎因子iNOS、IL-6含量显著降低(P<0.05),IL-6、p-JAK2、p-STAT3表达量显著减少(P<0.05)。结论番泻苷B具有抑制胃癌细胞SGC-7901生长、干样特性、炎症因子水平以及诱导肿瘤细胞凋亡的作用,其机制可能与下调IL-6/JAK2/STAT3信号通路活性有关,从而缓解胃癌的发生发展。This study was designed to investigate the effects of senoside B on the growth,apoptosis,stem-like properties and IL-6/JAK2/STAT3 pathway of gastric cancer cells.The activity of gastric cancer cell SGC-7901 was detected by CCK8 method,while the apoptosis of SGC-7901 was detected by flow cytometry.The pellet formation of stem cells was observed by microscopy;CD133+stem cells were sorted by flow cytometry.Western blot was used to detect stem cell markers OCT4 and SOX2,while ELISA for the levels of inflammatory factors iNOS,IL-6,IL-4.Furthermore,Western blot also used to detect the expression of IL-6/JAK2/STAT3 pathway related proteins IL-6,p-JAK2,p-STAT3.CCK8 assay showed that the inhibitory effect of senoside B on SGC-7901 cell growth was significant(P<0.05),and 4 dose groups of senoside B(0,5,10,20μmol·L-1)were screened out for subsequent experiments.Compared with 0μmol·L-1 dose group,5μmol·L-1 dose group has no significant difference,while 10,20μmol·L-1 dose groups demonstrated higher level of apoptosis rate and anti-inflammatory factor IL-4(P<0.05),lower level of stem cell pellet formation and pellet diameter,as well as lower level of CD133+,OCT4,SOX2,iNOS,IL-6,p-JAK2,and p-STAT3(P<0.05).These results indicated that senoside B could inhibit the growth,the stem-like properties and inflammatory factors levels of gastric cancer cells SGC-7901,and induce the apoptosis of tumor cells.The mechanism may be related to the down-regulation of IL-6/JAK2/STAT3 signaling pathway activity,thus postponing the occurrence and development of gastric cancer.

关 键 词：番泻苷B 胃癌 SGC-7901细胞 干样特性 

分 类 号：R735.2[医药卫生—肿瘤]