6S-5-甲基四氢叶酸钙对小鼠免疫力的增强作用研究  

Research on the enhancement effect of 6S-5-methyltetrahydrofolate calcium on the immunity of mice

作　　者：薛娟 马文斌 涂华 刘康 成永之 连增林 XUE Juan;MA Wenbin;TU Hua;LIU Kang;CHENG Yongzhi;LIAN Zenglin(Beijing Jinkang Hexin Pharmacy Technology Co.,Ltd,Beijing 100176,China;Lianyungang Jinkang Hexin Pharmacy Co.,Ltd,Jiangsu,Lianyungang 222000,China)

机构地区：[1]北京金康和信药业科技有限公司,北京100176 [2]连云港金康和信药业有限公司,江苏连云港222000

出　　处：《中国医药科学》2021年第17期29-33,共5页China Medicine And Pharmacy

摘　　要：目的观察6S-5-甲基四氢叶酸钙对小鼠免疫力的影响。方法将小鼠分为低剂量组、中剂量组、高剂量组以及阴性对照组,每组各10只,低、中、高剂量组分别灌胃给予0.8 mg/(kg·d)、1.7 mg/(kg·d)、5.0 mg/(kg·d)6S-5-甲基四氢叶酸钙,阴性对照组给予同等容积的饮用纯净水,观察6S-5-甲基四氢叶酸钙对小鼠体重、胸腺/体重比、脾脏/体重比、小鼠脾淋巴细胞转化能力、迟发型超敏(DTH)反应、溶血空斑数、血清溶血素含量、碳廓清能力、巨噬细胞吞噬鸡红细胞能力和自然杀伤细胞(NK cells)活性的影响。结果与阴性对照组相比,受试三个剂量组小鼠体重差异无统计学意义(P>0.05),胸腺/体重比、脾脏/体重比较低(P>0.05),小鼠DTH程度增加(P>0.05);低、中、高剂量组小鼠碳廓清能力呈剂量依赖性增加(P<0.05),NK细胞活性明显增强(P<0.05);中、高剂量组小鼠淋巴细胞增殖能力、溶血空斑数、抗体积数和巨噬细胞吞噬鸡红细胞能力均增加(P<0.05)。结论6S-5-甲基四氢叶酸钙能增强小鼠的免疫力。Objective To observe the impacts of 6S-5-methyltetrahydrofolate calcium on the immunity of mice.Methods The mice were divided into the low dose group,the medium dose group,the high dose group and the negative control group,with 10 mice in each group.The low,medium and high dose groups were treated with 6S-5-methyltetrahydrofolate calcium of 0.8 mg/(kg·d),1.7 mg/(kg·d)and 5.0 mg/(kg·d)by gavage respectively,while the negative control group was treated with the same volume of purified drinking water.The impacts of 6S-5-methyltetrahydrofolate calcium on body weight,thymus/body weight ratio,spleen/body weight ratio,spleen lymphocyte transformation ability,delayed type hypersensitivity(DTH)reaction,hemolytic plaque number,serum hemolysin content,carbon clearance ability,macrophage phagocytosis of chicken red blood cells and natural killer(NK)cells activity in mice were observed.Results Compared with the negative control group,there was no significant difference in body weight among the three doses groups(P>0.05),the ratios of thymus/body weight and spleen/body weight were relatively low(P>0.05),and the degree of DTH in mice increased(P>0.05).In the low,medium and high doses groups,the carbon clearance ability of mice increased in a dose-dependent manner(P<0.05),and the NK cell activity increased significantly(P<0.05).The ability of lymphocyte proliferation,hemolytic plaque number,antibody product number and macrophage phagocytic ability of chicken red blood cells increased in the medium and high doses groups(P<0.05).Conclusion 6S-5-methyltetrahydrofolate calcium can enhance the immunity o f mice.

关 键 词：6S-5-甲基四氢叶酸钙 增强免疫力 抗体生成细胞检测实验 血清溶血素测定 碳廓清实验 

分 类 号：R-332[医药卫生]