PTOV1对食管鳞癌细胞系增殖和迁移的影响  

Effect of PTOV1 on proliferation and migration of esophageal squamous cell carcinoma cell line

作　　者：张彦秋 文珍 吴蓉 陈彬 冷爱民[1] Zhang Yanqiu;Wen Zhen;Wu Rong;Chen Bin;Leng Aimin(Department of Gastroenterology,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Internal Medicine,People′s Hospital of Maerkang City,Aba Prefecture,Maerkang 624000,China)

机构地区：[1]中南大学湘雅医院消化内科,长沙410008 [2]四川省阿坝州马尔康市人民医院内科,624000

出　　处：《中国医师杂志》2021年第9期1336-1341,共6页Journal of Chinese Physician

基　　金：湖南省科技创新计划项目(2018SK52603)。

摘　　要：目的探讨沉默及过表达前列腺肿瘤过表达1(PTOV1)后对食管鳞癌细胞系Ec9706和TE-1细胞增殖和迁移能力的影响,以探究PTOV1在食管癌发生发展中的作用。方法体外培养食管鳞癌细胞系。使用慢病毒转染来敲低Ec9706细胞中PTOV1表达量,上调TE-1细胞系中PTOV1表达量,并使用Western blot检验上述细胞系中PTOV1的表达。分别采用划痕实验、Transwell细胞迁移实验、CCK8实验和集落形成实验检测不同PTOV1表达水平的同种食管鳞癌细胞增殖和迁移能力的差异。结果经Western blot检测,Ec9706细胞经慢病毒转染后PTOV1表达较空载体组显著降低,TE-1经过慢病毒转染后PTOV1表达较空载体组显著升高,证实转染成功。沉默Ec9706细胞系中PTOV1的表达后,细胞的迁移率、穿膜细胞数、增殖能力、细胞集落形成率均较空载体组下降(P<0.05)。而过表达TE-1细胞系中PTOV1后,细胞的迁移率、穿膜细胞数、增殖能力、细胞集落形成率均较空载体组增加(P<0.05)。结论PTOV1可促进食管鳞癌细胞系的增殖和迁移能力。Objective To investigate the effects of silencing and overexpression of prostate tumor overexpressed 1(PTOV1)on the proliferation and migration of esophageal squamous cell lines Ec9706 and TE-1,in order to explore the role of PTOV1 in the development of esophageal cancer.Methods Esophageal squamous cell carcinoma cell lines were cultured in vitro.Lentivirus transfection was used to knock down the expression of PTOV1 in Ec9706 cells,up regulate the expression of PTOV1 in TE-1 cell lines.The expression of PTOV1 in the above cell lines was detected by Western blot.Scratch test,transwell cell migration test,cell counting kit-8(CCK8)test and colony formation test were used to study the difference of proliferation and migration ability of homologous esophageal squamous cell carcinoma cells with different expression levels of PTOV1.Results Western blot showed that the expression of PTOV1 in Ec9706 cells transfected with lentivirus was significantly lower than that in the empty vector group,and the expression of PTOV1 in TE-1 cells transfected with lentivirus was significantly higher than that in the empty vector group,which confirmed that the transfection was successful.After silencing the expression of PTOV1 in Ec9706 cell line,the cell migration rate,the number of transmembrane cells,proliferation ability and colony formation rate decreased compared with the empty vector group(P<0.05).After overexpression of PTOV1 in TE-1 cell line,the cell migration rate,the number of transmembrane cells,proliferation ability and colony formation rate were higher than those in empty vector group(P<0.05).Conclusions PTOV1 can promote cell proliferation and migration in esophageal squamous cell cancer(ESCC)cell line.

关 键 词：食管肿瘤 癌 鳞状细胞 PTOV1 细胞增殖 细胞迁移 

分 类 号：R735.1[医药卫生—肿瘤]