氧苯酮对龙须菜(Gracilaria lemaneiformis)光合作用和呼吸作用的影响  

Effect of oxybenzone on the photosynthesis and respiration of Gracilaria lemaneiformis

作　　者：田琳[1] 李祥蕾 TIAN Lin;LI Xiang-lei(College of Environmental Science&Engineering,Ocean University of China,Qingdao 266100,China;Yantai Center of Ecology and Environment Monitoring of Shandong Province,Yantai 264000,China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]山东省烟台生态环境监测中心,山东烟台264000

出　　处：《海洋环境科学》2021年第5期760-765,共6页Marine Environmental Science

基　　金：江苏省高校自然科学基金项目(19KJB170010)。

摘　　要：本文以野生龙须菜(Gracilaria lemaneiformis)为受试材料,测定氧苯酮(BP-3)胁迫下龙须菜的快速叶绿素荧光、光合放氧/呼吸耗氧速率及活性氧含量的变化,研究了不同浓度的氧苯酮对龙须菜光合作用和呼吸作用的影响,分析了氧苯酮对水生植物的伤害机理。结果表明,用5~30μmol/L的BP-3在黑暗条件下处理30 h后,龙须菜的呼吸作用不受影响,但光合作用PSII受体侧电子传递受到明显抑制。在80μmol/(m^(2)·s)光下,5~30μmol/L的BP-3可导致光合作用光能吸收与利用的失衡并诱发活性氧的大量产生,且抑制作用随胁迫浓度升高而加剧。活性氧测定结果进一步显示,光下BP-3诱导的氧化胁迫加剧了龙须菜光合作用PSII受体侧的破坏,并进一步损伤其呼吸作用过程和光合作用PSII供体侧。The effect of oxybenzone(BP-3) on the photosynthesis and respiration of wild Gracilaria lemaneiformis was investigated by determining the response of the rapid chlorophyll fluorescence,photosynthetic oxygen evolution rate, respiratory oxygen uptake rate, and active oxygen content in this alga exposed to 0 to 30 μmol/L of this reagent. Results showed that 5~30 mol/L BP-3 had a slight effect on respiration after 30 hours treatment without light, but could directly inhibit the electron transfer of PSII receptor side of photosynthesis in G. lemaneiformis. At 80 μmol/m^(2)/s light, BP-3 caused an imbalance of photosynthetic light energy absorption and utilization, and further induced the over-production of reactive oxygen species. The inhibition was intensified with the increase of BP-3 concentration. The oxidative stress induced by BP-3 would aggravate the damage of PSII receptor side of photosynthesis, and further damage the respiration process and photosynthetic PSII donor side in the seaweed.

关 键 词：氧苯酮 龙须菜 光合作用 呼吸作用 光合电子传递 

分 类 号：X171.5[环境科学与工程—环境科学]