p16、微型染色体维持蛋白2、Ki-67在宫颈鳞状上皮内病变的表达与意义  

Expression and significance of p16,mini-chromosome maintenance protein 2 and Ki-67 in cervical squamous intraepithelial lesions

作　　者：蒋莉萍[1] 吴凤珍[2] 肖艳 胡利娟[1] 刘媛媛 JIANG Liping;WU Fengzhen;XIAO Yan;HU Lijuan;LIU Yuanyuan(Department of Pathology,Ji'an Maternity and Child Health Hospital;Department of Obstetrics and Gynecology,Ji’an Maternity and Child Health Hospital,Ji’an,Jiangxi 343006,China)

机构地区：[1]吉安市妇幼保健院病理科 [2]吉安市妇幼保健院妇产科,江西吉安343006

出　　处：《现代医学与健康研究电子杂志》2021年第17期35-38,共4页Modern Medicine and Health Research

基　　金：吉安市科技计划项目[吉安市科计字(2019)8号-89]。

摘　　要：目的检测子宫颈鳞状上皮内病变(SIL)组织中p16、微型染色体维持蛋白2(MCM2)、Ki-67的表达,分析三者阳性表达的关系,为疾病的临床诊断与治疗提供依据。方法回顾性分析吉安市妇幼保健院2017年7月至2019年10月收治的240例宫颈上皮内瘤变(CIN)患者(CINⅠ级患者130例,CINⅡ~Ⅲ级110例患者)、50例慢性宫颈炎患者及20例宫颈浸润性鳞癌患者的临床资料。采集各患者宫颈活检组织,进行SP法免疫组织化学染色,检测各患者子宫颈病理组织p16、MCM2、Ki-67的表达情况,分析p16、MCM2、Ki-67阳性表达的相关性。结果CINⅠ级、CINⅡ~Ⅲ级、浸润性鳞癌患者病理组织中p16、MCM2、Ki-67阳性表达率均显著高于慢性宫颈炎患者,且CINⅡ~Ⅲ级、浸润性鳞癌患者显著高于CINⅠ级患者;Spearman相关性分析显示,p16与MCM2阳性表达呈正相关关系(r=0.691);p16与Ki-67阳性表达呈正相关(r=0.556);MCM2与Ki-67阳性表达呈正相关(r=0.676)(均P<0.05)。结论在慢性宫颈炎患者、CIN患者及浸润性鳞癌患者宫颈组织中p16、MCM2、Ki-67均异常高表达,且三者阳性表达与疾病严重程度正相关,p16、MCM2、Ki-67三者阳性表达也具有显著正相关性,可将其作为SIL诊断的分子标志物。Objective To detect the expression of p16,mini-chromosome maintenance protein 2(MCM2)and Ki-67 in cervical squamous intraepithelial lesion(SIL)tissue,and analyze the relationship between the positive expressions among the three,provide basis for clinical diagnosis and treatment of diseases.Methods Retrospectively analysed of the clinical datas of the 240 cases patients with cervical intraepithelial neoplasia(CIN)(130 patients with CINⅠgrade,110 patients with CINⅡ~Ⅲgrade),50 patients with chronic cervicitis and 20 patients with invasive squamous cell carcinoma of cervix,who admitted to Ji'an Maternity and Child Health Hospital from July 2017 to October 2019.The cervical biopsy tissues of each patient were collected and subjected to SP immunohistochemical staining,the expression of p16,MCM2,and Ki-67 in the cervical pathological tissues of each patient were detected,and analyze the correlation of the positive expression of p16,MCM2,and Ki-67.Results The positive expression rates of p16,MCM2,and Ki-67 in the pathological tissues of patients with CIN gradeⅠ,CIN gradeⅡ~Ⅲ,and invasive squamous cell carcinoma of cervix were significantly higher than those of chronic cervix inflammation patients,and patients with CIN gradeⅡ~Ⅲ,invasive squamous cell carcinoma of cervix were significantly higher than patients with CIN gradeⅠ;Spearman correlation analysis showed that,the positive expression of p16 and MCM2 were positively correlated(r=0.691);the positive expression of p16 and Ki-67 were positively correlated(r=0.556);the positive expression of MCM2 and Ki-67 were positively correlated(r=0.676)(all P<0.05).Conclusion p16,MCM2,and Ki-67 in patients with chronic cervicitis,CIN patients and patients with invasive squamous cell carcinoma of cervix are all abnormally highly expressed,and the positive expression of p16,MCM2,and Ki-67 positively correlated with the severity of the disease,p16,MCM2,and Ki-67 positive expression also has a significant positive correlation,which can be used as the molecular markers f

关 键 词：P16 微型染色体维持蛋白2 KI-67 宫颈鳞状上皮内病变 

分 类 号：R737.33[医药卫生—肿瘤]