长链非编码RNA核富集转录体1在甲状腺乳头状癌组织的表达及其临床意义  

Expression of lncRNA nuclear enrich abundant transcript 1 in thyroid papillary carcinoma and its clinical significance

作　　者：李建华[1] 段彩纹 徐鹏飞 张素琴[1] 郑守华[1] 邱新光[1] Li Jianhua;Duan Caiwen;Xu Pengfei;Zhang Suqin;Zheng Shouhua;Qiu Xinguang(Department of Thyroid Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院甲状腺外科,450052

出　　处：《中华实验外科杂志》2021年第10期1990-1993,共4页Chinese Journal of Experimental Surgery

基　　金：河南省科技攻关项目(201702067、202102310391、202102310437);河南省省部共建青年项目(SB201902003);河南省自然科学基金(202300410451)。

摘　　要：目的观察甲状腺乳头状癌(PTC)组织中长链非编码RNA核富集转录体1(lncRNA NEAT1)表达,探讨其机制及其临床意义。方法选取甲状腺乳头状癌根治手术的患者65例,应用实时定量聚合酶链反应(Real-time PCR)检测PTC组织和癌旁正常组织lncRNA NEAT1表达水平。应用独立样本t检验比较PTC组织和癌旁正常组织lncRNA NEAT1表达水平;应用受试者工作特征(ROC)曲线法分析lncRNA NEAT1对PTC的诊断效能;应用二元Logistic回归分析lncRNA NEAT1相关的独立危险因素;采用t检验和Mann-WhitneyU秩检验分析PTC组织中lncRNA NEAT1表达水平和临床病理特征的相关性。结果 PTC组织lncRNA NEAT1表达水平(1.640±0.221)高于癌旁正常组织(1.332±0.191),差异有统计学意义(t=8.510,P<0.05);当以组织lncRNA NEAT1表达水平≥1.456作为诊断PTC的标准,此时敏感度和特异度分别为81.5%、76.9%,约登指数为58.4%;Logistic回归分析显示,淋巴结转移、肿瘤局部侵犯等代表PTC不良预后的因素是组织lncRNA NEAT1检测结果的独立影响因子[比值比(OR):2.101、3.800,P<0.05、0.01]。PTC肿瘤淋巴结出现癌转移和肿瘤局部侵犯者,较淋巴结未出现癌转移和无肿瘤局部侵犯者组织lncRNA NEAT1表达水平升高,差异有统计学意义(淋巴结癌转移,t=3.136,P<0.05;肿瘤局部侵犯,t=3.008,P<0.05)。结论高表达的lncRNA NEAT1可能是PTC发生的促进因素,PTC组织lncRNA NEAT1表达水平升高提示其预后不良。Objective To investigate the expression,potential mechanism and clinical significance of long non-coding RNA nuclear enrich abundant transcript 1(lncRNA NEAT1)in papillary thyroid carcinoma(PTC).Methods A total of 65 patients diagnosed with PTC and received radical thyroidectomy were analyzed.Real-time quantitative polymerase chain reaction was used to detect lncRNA NEAT1 expression levels in PTC tissues and adjacent normal tissues.The expression level of lncRNA NEAT1 in PTC tissues and adjacent normal tissues was compared by independent sample t-test.The diagnostic efficacy of lncRNA NEAT1 for PTC was analyzed by receiver operating characteristic(ROC)curve method.Binary Logistic regression was used to analyze the independent risk factors associated with lncRNA NEAT1.t-test or Mann-Whitney U rank test were used to analyze the correlation between lncRNA NEAT1 expression level and clinicopathological characteristics in PTC tissues.Results The expression level of lncRNA NEAT1 in PTC tissues(1.640±0.221)was higher than that in adjacent normal tissues(1.332±0.191),and the difference was statistically significant.(t=8.510,P<0.05).When tissue lncRNA NEAT1 expression level 1.456 was used as the diagnostic criteria for PTC,the sensitivity and specificity were 81.5%and 76.9%respectively,The Youden index was 58.4%.Logistic regression analysis showed that factors representing poor prognosis of PTC,such as lymph node metastasis and local tumor invasion,were independent influencing factors for the results of lncRNA NEAT1 detection[odds ratio(OR):2.101,3.800,P<0.05,0.01].The lncRNA NEAT1 expression level of PTC tissues with pathological lymphatic metastasis,and tumor’s invasion increased,as opposed to PTC tissues without these features.The difference was statistically significant(lymphatic metastasis,t=3.136,P<0.05,invasion of tumor,t=3.008,P<0.05).Conclusion The high expression of lncRNA NEAT1 may be a promoting factor for the occurrence of PTC,and the elevated expression of lncRNA NEAT1 in PTC tissue suggests a poor progn

关 键 词：长链非编码RNA核富集转录体1 甲状腺乳头状癌 预后 

分 类 号：R736.1[医药卫生—肿瘤]