前列腺素类降眼压药物的长期使用对兔眼球结膜厚度的影响  

Effect of long-term topical administration of prostaglandins on bulbar conjunctival thickness in rabbits

作　　者：麻凯 殷成娟 张振永[1] Ma Kai;Yin Chengjuan;Zhang Zhenyong(Department of Ophthalmology,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China)

机构地区：[1]上海中医药大学附属普陀医院眼科,200062

出　　处：《中华实验眼科杂志》2021年第10期857-862,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：上海中医药大学附属普陀医院院级课题项目(2017301A);上海市第六人民医院联合体课题项目(18-LY-03)。

摘　　要：目的探讨前列腺素类降眼压药物的长期使用对兔眼球结膜厚度的影响及其可能的机制。方法采用随机数表法将24只清洁级新西兰大白兔随机分为拉坦前列腺素组、盐酸卡替洛尔组和空白对照组,每组8只,均取左眼作为实验眼,其中前2个组分别采用拉坦前列腺素滴眼液和盐酸卡替洛尔滴眼液点眼,每天1次,每次1滴,持续2个月;空白对照组不做任何处理。点眼前及点眼后2个月,采用光相干断层扫描(OCT)分别测量拉坦前列腺素组和盐酸卡替洛尔组实验兔左眼球结膜全层厚度。采用实时荧光定量PCR和Western blot法检测点眼后2个月各组实验兔球结膜组织中基质金属蛋白酶(MMP)-1和MMP-3 mRNA及蛋白表达水平变化。结果OCT检测结果显示,点眼前拉坦前列腺素组和盐酸卡替洛尔组球结膜厚度分别为(178.88±5.23)μm和(184.94±11.85)μm,点眼后2个月分别为(124.19±11.29)μm和(183.31±8.71)μm;点眼后2个月拉坦前列腺素组兔眼球结膜全层厚度较点眼前及盐酸卡替洛尔组点眼后2个月明显变薄,差异均有统计学意义(均P<0.01)。实时荧光定量PCR和Western blot检测结果显示,拉坦前列腺素组MMP-1和MMP-3 mRNA及蛋白相对表达量均较空白对照组和盐酸卡替洛尔组明显升高,差异均有统计学意义(均P<0.01)。结论长期点用前列腺素类滴眼液可使兔眼球结膜厚度明显变薄,其机制可能与球结膜组织中MMP-1和MMP-3表达水平升高有关。Objective To investigate the effect of long-term application of prostaglandin analog drops on bulbar conjunctival thickness in rabbits.Methods Twenty-four healthy New Zealand white rabbits were randomly divided into latanoprost group,carteolol group and blank control group using the random number table method,with 8 rabbits in each group.The left eyes of rabbits were taken as experimental eyes.The rabbits in the latanoprost group and carteolol group were given latanoprost eye drops or carteolol eye drops once a day for 2 months according to grouping.The bulbar conjunctival thickness of left eyes of the latanoprost group and carteolol group were measured by optical coherence tomography(OCT)at baseline and two months after administration,respectively.The conjunctival tissue of the three groups were extracted to investigate the protein and mRNA expression level of matrix metalloproteinases-1(MMP-1)and MMP-3 by Western blot and real-time fluorescence quantitative polymerase chain reaction(PCR).The study protocol was approved by an Ethics Committee of Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine(No.2017-0014).The use and care of the experimental animals complied with the ARVO Statement.Results In the latanoprost group,the conjunctival thickness was significantly reduced from baseline(178.88±5.23)pm to(124.19±11.29)pm at 2 months after administration(P<0.01).In the carteolol group,there existed no significant difference in the conjunctival thickness between baseline(184.94±11.85)Rm and(183.31±8.71)Rm at 2 months after administration(P>0.05).The conjunctival thickness at 2 months after administration of the latanoprost group was significantly thinner than that of the carteolol group(P<0.01).The protein and mRNA expression levels of MMP-1 and MMP-3 in conjunctival tissue of the latanoprost group were significantly higher than those of the blank control group and carteolol group(all at P<0.01).Conclusions The long-term topical use of prostaglandin analog drops can significantly redu

关 键 词：前列腺素类药物 球结膜/厚度 基质金属蛋白酶 青光眼 滤过手术 

分 类 号：R775[医药卫生—眼科]