急性前葡萄膜炎患者血清IL-9及其他Th细胞相关因子水平的变化及临床意义  

Changes and clinical significance of serum interleukin-9 and other T helper cell-related cytokines in patients with acute anterior uveitis

作　　者：白惠玲[1] 樊爱芳 刘勤[1] 张书[1] 王芃萱 Bai Huiling;Fan Aifang;Liu Qin;Zhang Shu;Wang Pengxuan(Department of Ophthalmology,Gansu Provincial Hospital,Lanzhou 730000,China;First School of Clinical Medical of Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃省人民医院眼科,兰州730000 [2]甘肃中医药大学第一临床医学院,兰州730000

出　　处：《中华实验眼科杂志》2021年第10期880-884,共5页Chinese Journal Of Experimental Ophthalmology

摘　　要：目的探讨白细胞介素(IL)-9与其他辅助性T(Th)细胞相关因子在急性前葡萄膜炎发病中的作用。方法采用横断面研究,纳入2018年5月至2019年5月在甘肃省人民医院就诊的急性前葡萄膜炎患者36例36眼及同期与之相匹配的健康体检者40例40眼,分别作为急性前葡萄膜炎组和健康对照组;根据患者症状对病情严重程度进行分级并分为急性前葡萄膜炎患者轻度组、中度组和重度组。收集所有受检者血清,采用酶联免疫吸附测定(ELISA)法测定血清中IL-9、γ干扰素(IFN-γ)、IL-4、IL-17、转化生长因子-β1(TGF-β1)、IL-35和IL-22的质量浓度,并分析IL-9质量浓度与其他Th细胞因子质量浓度间的关系。结果急性前葡萄膜炎组患者血清中IL-9、IFN-γ、IL-4、TGF-β1、IL-35、IL-22质量浓度明显高于健康对照组,差异均有统计学意义(均P<0.05);2个组间血清中IL-17质量浓度差异无统计学意义(U=704.500,P=0.872)。急性前葡萄膜炎患者轻度组、中度组和重度组血清中IL-9质量浓度分别为57.24(47.47,65.10)、71.68(67.55,78.91)和114.01(74.78,139.30)ng/L,总体比较差异有统计学意义(Z=8.766,P=0.012);其中轻度组和中度组IL-9质量浓度明显低于重度组,差异均有统计学意义(均P<0.05)。急性前葡萄膜炎患者血清中IL-9质量浓度与IL-17、TGF-β1、IL-35质量浓度均呈正相关(rs=0.449、0.517、0.400,均P<0.05),与IFN-γ、IL-4、IL-22质量浓度均无明显相关性(rs=0.293、0.286、0.316,均P>0.05)。结论IL-9在急性前葡萄膜炎的发生和发展中具有促进免疫炎症反应的作用,其与Th17和Treg细胞相关因子(TGF-β1、IL-35)密切相关。Objective To explore the role of interleukin(IL)-9 and other T helper(Th)cell-related cytokines in the pathogenesis of acute anterior uveitis.Methods A cross-sectional study was conducted.Thirty-six patients(36 eyes)with acute anterior uveitis who were treated at Gansu Provincial Hospital from May 2018 to May 2019 and 40 matched healthy subjects(40 eyes)who had no eye diseases or systemic diseases in the same period were enrolled as the acute anterior uveitis group and healthy control group,respectively.The disease severity of the subjects in the acute anterior uveitis group was graded and the subjects were divided into mild,moderate and severe groups according to the grading.Serum of all subjects was collected to determine the concentration of serum IL-9,IL-17,transforming growth factor-β1(TGF-β1),interferon-γ(IFN-γ),IL-4,IL-35 and IL-22 by enzyme linked immunosorbent assay(ELISA)method.Spearman rank correlation analysis was used to evaluate the relationship between IL-9 concentration and other Th cell-related cytokines.This study adhered to the Declaration of Helsinki.The study protocol was approved by an Ethics Committee of Gansu Provincial Hospital(No.2019-204).Written informed consent was obtained from each subject prior to any medical examination.Results The serum levels of IL-9,IFN-γ,IL-4,TGF-β1,IL-35 and IL-22 in the acute anterior uveitis group were significantly higher than those in the healthy control group,and the differences were statistically significant(all at P<0.05).There was no statistically significant difference in the concentration of IL-17 between the two groups(U=704.500,P=0.872).The IL-9 concentration of patients with acute anterior uveitis in the mild,moderate and severe groups was 57.24(47.47,65.10),71.68(67.55,78.91)and 114.01(74.78,139.30)ng/L,respectively,and the overall difference was statistically significant(Z=8.766,P=0.012),and the IL-9 concentration of the mild group and the moderate group was significantly lower than that of the severe group(both at P<0.05).The concentrati

关 键 词：前葡萄膜炎 急性 白细胞介素-9 辅助性T细胞 细胞因子 

分 类 号：R773.9[医药卫生—眼科]