铁皮石斛醋酸乙酯部位化学成分研究  

Study on chemical constituents from active ethyl acetate fraction of Dendrobium officinale

作　　者：周宇娟 王俊豪 薛亚甫 徐红[1] 侴桂新[1] 王峥涛[1] ZHOU Yu-juan;WANG Jun-hao;XUE Ya-fu;XU Hong;CHOU Gui-xin;WANG Zheng-tao(Shanghai R&D Center for Standardization of Chinese Medicines,The State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Medicine,The Ministry of Education Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学中药研究所,教育部中药标准化重点实验室,中药新资源与品质评价国家中医药管理局重点研究室,上海中药标准化研究中心,上海201203

出　　处：《中草药》2021年第17期5218-5225,共8页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划(2017YFC1700801);国家自然科学基金资助项目(U19A2009);云南省王峥涛专家工作站(2018IC146)。

摘　　要：目的进一步研究铁皮石斛Dendrobium officinale活性醋酸乙酯部位的化学成分。方法运用大孔树脂、MCI、硅胶、凝胶(Sephadex LH-20)、ODS等柱色谱、制备薄层色谱及制备高效液相色谱法等分离技术,进行化合物的分离纯化,根据^(1)H-NMR、^(13)C-NMR、MS等波谱数据和理化性质鉴定化合物的结构。结果从铁皮石斛乙醇提取物的醋酸乙酯部位分离得到了34个化合物,分别鉴定为moscatin(1)、黑麦草内酯(2)、柚皮素(3)、橙皮苷(4)、甘草素(5)、2-(4-hydroxy-3-methoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-l-propanol(6)、异甘草素(7)、(E)-4-hydroxycinnamic acid(8)、(Z)-4-hydroxycinnamic acid(9)、coniferyl p-coumarate(10)、sinapyl p-coumarate(11)、tinosporaic acid A(12)、N-trans-coumaroyltyramine(13)、N-trans-feruloyltyramine(14)、丁香脂素(15)、pinoresinol(16)、梣皮树脂醇(17)、lirioresionol(18)、2(5H)-Furanone 5-hydroxy-3,4-dimethyl-5-pentyl(19)、丁香酸(20)、十六烷酸(21)、对羟基苯甲酸(22)、对羟基苯甲醛(23)、阿魏酸(24)、二氢松柏醇(25)、十七烷(26)、4-羟基-3,5-二甲氧基反式肉桂醛(27)、dihydro-p-cinnamic acid(28)、(+)-(4S)-(2E)-4-羟基-2-壬烯酸(29)、1,3-benzenediol(30)、对羟基苯酚(31)、水杨酸(32)、对羟基苯乙酮(33)和香草醛(34)。结论化合物2首次从石斛属植物中分离得到,化合物3~7均为首次从铁皮石斛中分离得到。Objective To study the chemical constituents from the active ethyl acetate part of the stems of Dendrobium officinale.Methods The compounds were isolated and purified by column chromatographies on macroporous resin,MCI,silica gel,Sephadex LH-20,ODS,preparative thin-layer chromatography and preparative RP-HPLC.Their structures were identified by the analysis of their spectra data of ^(1)H-NMR,^(13)C-NMR,MS and the physical and physiochemical properties.Results A total of 34 compounds were isolated and identified from the ethyl acetate fraction of D.officinale as moscatin(1),loliolide(2),naringin(3),hesperetin(4),liquiritigenin(5),2-(4-hydroxy-3-methoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-l-propanol(6),isoliquiritigenin(7),(E)-4-hydroxycinnamic acid(8),(Z)-4-hydroxycinnamic acid(9),coniferyl p-coumarate(10),sinapyl p-coumarate(11),tinosporaic acid A(12),N-trans-coumaroyltyramine(13),N-trans-feruloyltyramine(14),syringaresinol(15),pinoresinol(16),medioresinol(17),lirioresionol(18),2(5H)-furanone 5-hydroxy-3,4-dimethyl-5-pentyl(19),syringic acid(20),palmitic acid(21),4-hydroxybenzoic acid(22),p-hydroxybenzaldehyde(23),ferulic acid(24),dihydroconiferyl alcohol(25),N-heptadecane(26),4-hydroxy-3,5-dimethoxy trans cinnamaldehyde(27),dihydro-p-cinnamic acid(28),(+)-(4S)-(2E)-4-hydroxy-2-nonanoic acid(29),1,3-benzenediol(30),hydroquinone(31),salicylic acid(32),p-hydroxyacetophenone(33),and vanillin(34).Conclusion Compound 2 is isolated from Dendrobium for the first time,and compounds 3-7 are found from D.officinale for the first time.

关 键 词：铁皮石斛 loliolide 柚皮素 HESPERETIN 甘草素 2-(4-hydroxy-3-methoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-l-propanol 异甘草素 

分 类 号：R284.1[医药卫生—中药学]