湖栖鳍虾虎鱼皮肤和眼睛转录组比较  

Comparative analysis of skin and eye transcriptome in the Gobiopterus lacustris

作　　者：黎学友 谢明花 黄承勤 汪淳 王中铎[1] 郭昱嵩[1] 董忠典 LI Xueyou;XIE Minghua;HUANG Chengqin;WANG Chun;WANG Zhongduo;GUO Yusong;DONG Zhongdian(Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,南海水产经济动物增养殖重点实验室,广东湛江524088

出　　处：《水产学报》2021年第8期1317-1326,共10页Journal of Fisheries of China

基　　金：国家自然科学基金(41806195,31201996);广东省科技厅项目(2017A030303075);广东海洋大学创新强校项目(230419069,230419055);广东海洋大学“南海学者计划”青年人才项目(QNXZ201903,201807);广东海洋大学博士启动项目。

摘　　要：为了对湖栖鳍虾虎鱼的皮肤和眼睛进行转录组比较分析,实验通过测序原始数据,经de novo拼接、组装共获得103686个单基因(unigene),N50和平均长度分别为1456和2490 bp。在Nr、Nt、KO、SwissProt、PFAM、GO及KOG数据库中对上述unigenes进行注释,分别有57380、37343、31700、51277、47020、47555和25604个unigenes获得注释。对差异基因进行KEGG富集发现,在黑色素生成途径中,tyr、tyrp1和tyrp2等基因在皮肤中明显下调。基因表达差异分析显示,在湖栖鳍虾虎鱼皮肤和眼睛中共有8113个差异表达基因(DEGs),其中3174个DEGs在皮肤中上调,4939个DEGs在眼睛中上调。通过实时荧光定量PCR(qPCR)对10个DEGs的验证,确定了RNA-Seq分析正确。本研究丰富了湖栖鳍虾虎鱼的体色研究,为湖栖鳍虾虎鱼遗传资源的利用提供了数据,也为进一步研究鱼类绚丽的体色形成机制提供了参考。Gobiopterus lacustris is a kind of small fish with strong adaptability to salinity and easy to raise in laboratory.It is an ideal model organism and environmental indicator species,whose body is transparent and its organs can be seen through naked eyes.In order to explore the cause of the transparent body color of G.lacustris,the transcriptome analysis of skin and eye of G.lacustris was carried out.A total of 103686 unigenes were obtained by de novo splicing.The N50 and average length were 1456 and 2490 bp,respectively.In Nr,Nt,KO,SwissProt,PFAM,GO and KOG databases,57380,37343,31700,51277,47020,47555 and 25604 unigenes were annotated respectively.KEGG enrichment showed that tyr,tyrp1 and tyrp2 were significantly down regulated in melanin production pathway.In addition,the results showed that there were 8113 differentially expressed genes(DEGs)in the skin and eyes of G.lacustris,of which 3174 were up-regulated in the skin and 4939 in the eyes.The verification of 10 DEGs by qPCR confirmed that the RNA-Seq analysis was correct.This study enriches the body color research of G.lacustris,provides data for the utilization of G.lacustris genetic resources,and also provides a reference for further study on the formation mechanism of gorgeous body color of fish.

关 键 词：湖栖鳍虾虎鱼 皮肤 眼睛 RNA-SEQ 色素形成 体色 

分 类 号：S917.4[农业科学—水产科学]