细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1免疫组织化学染色指标在非小细胞肺癌鉴别诊断中的应用  

Application of immunohistochemical staining indexes of cytokeratin 5/6,P63 protein,cytokeratin 7,thyroid transcription factor 1 in the differential diagnosis of non-small cell lung cancer

作　　者：李飞妹 王武明[2] 赵龙 LI Fei-mei;WANG Wu-ming;ZHAO Long(Department of Pathology,Jiangxi Chest Hospital,Jiangxi Province,Nanchang330000,China;Department of Thoracic Surgery,Jiangxi Chest Hospital,Jiangxi Province,Nanchang330000,China)

机构地区：[1]江西省胸科医院病理科,江西南昌330000 [2]江西省胸科医院胸外科,江西南昌330000

出　　处：《中国当代医药》2021年第29期98-100,共3页China Modern Medicine

基　　金：江西省卫生健康委科技计划项目(202130972)。

摘　　要：目的研究非小细胞肺癌患者采用细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1免疫组织化学染色指标检测方式对病情进行诊断的效果。方法选择2018年10月至2020年10月在江西省胸科医院治疗后证实为非小细胞肺癌、慢性阻塞性肺疾病患者的肺部病变组织支气管镜活检切片,各40例,再抽取上述肺病患者的肺部正常组织支气管镜活检切片40例,分别将其定义为研究1组、研究2组、对照组。采用免疫组织化学染色法对三组研究对象的细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1四项指标的阳性表达情况进行测定,比较三组研究对象的细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1四项指标水平的检测结果。结果研究1组研究对象的细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1四项指标阳性表达率高于研究2组,差异有统计学意义(P<0.05);研究2组研究对象的细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1四项指标阳性表达率高于对照组,差异有统计学意义(P<0.05)。结论非小细胞肺癌患者采用细胞角蛋白5/6、P63蛋白、细胞角蛋白7、甲状腺转录因子1四项免疫组织化学染色指标的阳性表达率高于慢性阻塞性肺疾病患者及健康人群,临床中可以将其作为非小细胞肺癌患者病情筛查和诊断的重要依据。Objective To study the effect of immunohistochemical staining indexes of cytokeratin 5/6,P63 protein,cytokeratin 7 and thyroid transcription factor 1 in the diagnosis of non-small cell lung cancer.Methods From October 2018 to October 2020,40 cases of bronchoscopic biopsy of lung lesions of patients with non-small cell lung cancer and 40 cases of bronchoscopic biopsy of lung lesions of patients with chronic obstructive pulmonary disease confirmed by treatment in Jiangxi Chest Hospital were selected,and 40 cases of bronchoscopic biopsy of normal lung tissue of patients were selected,which were divided into the study group 1,the study group 2 and the control group.Immunohistochemical staining was used to detect the positive expression of cytokeratin 5/6,P63 protein,cytokeratin 7 and thyroid transcription factor 1 in the three groups,and the detection results of the four indexes of cytokeratin 5/6,P63 protein,cytokeratin 7 and thyroid transcription factor 1 were compared among the three groups.Results The positive expression rates of cytokeratin 5/6,P63 protein,cytokeratin 7 and thyroid transcription factor 1 in the study group 1 were higher than those in the study group 2,the differences were statistically significant(P<0.05).The positive expression rates of cytokeratin 5/6,P63 protein,cytokeratin 7 and thyroid transcription factor 1 in the study group 2 were higher than those in the control group,the differences were statistically significant(P<0.05).Conclusion The positive expression rate of cytokeratin 5/6,P63 protein,cytokeratin 7 and thyroid transcription factor 1 in patients with non-small cell lung cancer are significantly higher than those in patients with chronic obstructive pulmonary disease and healthy people,which can be used as an important basis for screening and diagnosis of non-small cell lung cancer.

关 键 词：非小细胞肺癌 细胞角蛋白5/6 P63蛋白 细胞角蛋白7 甲状腺转录因子1 

分 类 号：R734.2[医药卫生—肿瘤]