t(8;21)急性髓系白血病细胞中HIF1α与WTAP表达关系研究  

The Relationship between HIF1α and WTAP Expression Level in t(8;21)Acute Myeloid Leukemia

作　　者：邵杨柳 陈泽 王莉莉 刘代红 高晓宁 SHAO Yang-Liu;CHEN Ze;WANG Li-Li;LIU Dai-Hong;GAO Xiao-Ning(Department of Hematology,First Medical Center,Chinese PLA General Hospital,Beijing 100853,China;Medical School of Chinese PLA,Beijing 100853;Chinese PLA Unit 31641,Guigang 537100,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]中国人民解放军总医院第一医学中心血液病科,北京100853 [2]解放军医学院,北京100853 [3]中国人民解放军31647部队,广西贵港537100

出　　处：《中国实验血液学杂志》2021年第5期1424-1428,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金(81870109、82070149);北京市自然科学基金(7202191)。

摘　　要：目的:探讨t(8;21)急性髓系白血病细胞中HIF1α与WTAP表达的关系。方法:用HIF1α特异性抑制剂棘霉素(Echinomycin)处理t(8;21)阳性急性髓系白血病细胞系SKNO-1、Kasumi-1 24 h,采用RT-q PCR及Western blot检测WTAP mRNA及蛋白的表达水平。经Co Cl2诱导细胞缺氧24 h,采用Western blot检测HIF1α、WTAP蛋白的表达水平。结果:Echinomycin处理组WTAP mRNA及蛋白表达水平显著低于对照组(P<0.01),Co Cl_(2)处理组WTAP蛋白表达水平显著高于对照组(P<0.05)。结论:抑制HIF1α可使WTAP的表达下调,而上调HIF1α则可使WTAP的表达上调,HIF1α与WTAP表达水平呈正相关,表明HIF1α可能参与了对WTAP的调控。Objective:To investigate the relationship between hypoxia inducible factor 1(HIF1α)and Wilms’tumor1-associating protein(WTAP)expression level in t(8;21)acute myeloid leukemia cells.Methods:The t(8;21)acute myeloid leukemia cell lines,including SKNO-1 and Kasumi-1 were treated by Echinomycin for 24 h,RT-qPCR and Western blot were used to detect the expression levels of WTAP mRNA and the protein.The CoCl2 was used to induce the hypoxia of the cells for 24 h,the expression levels of HIF1α,WTAP protein were detected by Western blot.Results:The expression level of WTAP mRNA and the protein in the echinomycin treated group was significantly lower than those in the control group(P<0.01).The expression level of WTAP protein in the CoCl2 treated group was significantly higher than those in the control group(P<0.05).Conclusion:The inhibition of HIF1-αcould downregulates the expression of WTAP,while the up-regulation of HIF1αcould up-regulates the expression of WTAP,which shows that there is a positive correlation of HIF1αand WTAP expression.This result suggesting that HIFla may be involves in the expression regulation of WTAP gene.

关 键 词：急性髓系白血病 t(8 21)染色体易位 缺氧诱导因子1 Wilms瘤1-关联蛋白 基因表达调控 

分 类 号：R733.71[医药卫生—肿瘤]