酶法制备富含中碳链甘油二酯的山苍子核仁油工艺研究  

Preparation of Litseacubeba Kernel Oil Rich in Medium-Chain Diacylglycerol Byenzymaticmethod

作　　者：李桥妹 王宗德[2] 谢礼文 黎冬明[1,2] 范国荣[2] 陈尚钘[2] Li Qiaomei;Wang Zongde;Xie Liwen;Li Dongming;Fan Guorong;Chen Shangxing(College of Food Science and Engineering,Jiangxi Agricultural University 1,Nanchang 330045;College of Forestry,Jiangxi Agricultural University,East China Woody Fragrance and Flavor Engineering Research Center of National Forestry and Grassland Administration,Camphor Engineering Research Center of NFGA/Jiangxi Province 2,Nanchang 330045)

机构地区：[1]江西农业大学食品科学与工程学院,南昌330045 [2]江西农业大学林学院,国家林草局木本香料(华东)工程技术研究中心,国家林草局/江西省樟树工程技术研究中心,南昌330045

出　　处：《中国粮油学报》2021年第8期54-59,共6页Journal of the Chinese Cereals and Oils Association

基　　金：“十三五”国家重点研发计划(2017YFD0600704)。

摘　　要：以山苍子核仁油为原料,筛选出最佳固定化脂肪酶催化不完全水解合成中碳链甘油二酯。在单因素试验的基础上,通过正交试验确定的最优反应条件为:加水量5%(占油重)、加酶量16%(占油重)、反应时间7 h。该条件下制备中碳链甘油二酯的质量分数为47.51%,而山苍子核仁原油中的中碳链甘油二酯的质量分数为17.24%,与山苍子核仁油原油中的中碳链甘油二酯的含量相比提高了2.76倍。此工艺为山苍子核仁油的工业化生产中碳链甘油二酯提供依据。Litseacubeba kernel oil was used as the raw material,and the best immobilized lipase was selected to catalyze the incomplete hydrolysis to synthesize medium-chain diacylglycerol.Based on single factor experiment,the optimal reaction conditions determined by the orthogonal experiment were as follows:water addition of 5%(based on oil weight),enzyme addition of 16%(based on oil weight),and reaction time of 7 hours.Under these conditions,the content of medium-chain diacylglycerol was 47.51%,while the content of medium-chain diacylglycerol in the crude oil of Litseacubeba kernel was 17.24%.Compared with the content of medium-chain diacylglycerol in crude oil of Litseacubeba kernel,it was increased by 2.76 times.This process provided a theoretical basis for the industrial production of medium-chain diacylglycerol in Litseacubeba kernel oil.

关 键 词：山苍子核仁油 固定化脂肪酶中碳链 甘油二酯 正交试验 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]