CD44、CD166在溃疡性结肠炎上皮内瘤变中的表达及临床意义  

Expressions and clinical significance of CD44 and CD166 in ulcerative colitis intraepithelial neoplasia

作　　者：李莹[1] 杨蔚峰[2] 宁燕华 黄芳菊 胡馨予 Li Ying;Yang Weifeng;Ning Yanhua;Huang Fangju;Hu Xinyu(Department of Pathology,The First People's Hospital of Qinzhou,Qinzhou 535000,China;Department of Gastroenterology,The First People's Hospital of Qinzhou,Qinzhou 535000,China)

机构地区：[1]广西壮族自治区钦州市第一人民医院病理科,535000 [2]广西壮族自治区钦州市第一人民医院消化内科,535000

出　　处：《国际医药卫生导报》2021年第20期3165-3169,共5页International Medicine and Health Guidance News

基　　金：钦州市科学研究与技术开发计划项目(20201611)。

摘　　要：目的分析CD44、CD166在溃疡性结肠炎(UC)上皮内瘤变(IN)中的表达,明确其在正常肠黏膜及UC-IN中的解剖特征,探讨其病理学意义。方法选取钦州市第一人民医院2018年10月至2021年1月UC-IN患者53例[男38例,女15例,年龄(47.95±7.16)岁]为研究对象,另选取同期正常肠黏膜组织者14例[男10例,女4例,年龄(49.05±7.31)岁],采集标本制成组织芯片,以免疫组化法检测CD44、CD166表达,通过免疫组化染色定位CD44、CD166阳性细胞,统计CD44、CD166染色结果。比较CD44、CD166在UC-IN及正常肠黏膜中的表达,分析不同病灶浸润深度、不同病理分级UC-IN患者CD44、CD166的临床表达。结果53例UC-IN患者CD44阳性细胞沿腺管基底侧呈小灶性弱阳性染色,CD166阳性细胞沿腺管基底侧呈黄色胞浆染色;14例正常肠黏膜组织者CD44均为阴性染色,CD166基本为阴性染色,部分可见腺管基底侧小灶性浅黄色胞浆染色。UC-IN患者CD44、CD166表达水平明显高于正常肠黏膜者(P<0.05)。病灶浸润至浆膜层的UC-IN患者CD44、CD166表达水平明显高于病灶浸润至肌层者(均P<0.05)。病理分级为中的UC-IN患者CD44、CD166表达水平低于病理分级为低的UC-IN患者(均P<0.05)。结论CD44、CD166在UC-IN患者中呈高表达,且与病灶浸润深度、病理分级关系密切,通过检测其水平对UC-IN临床诊疗、预后评估具有一定价值,有助于分析UC-IN患者病灶恶性病变风险,临床应用价值较高。Objective To analyze the expressions of CD44 and CD166 in ulcerative colitis(UC)intraepithelial neoplasia(IN),to clarify the anatomical characteristics of CD44 and CD166 in normal intestinal mucosa and UC-IN,and to explore their pathological significance.Methods A total of 53 UC-IN patients in The First People's Hospital of Qinzhou from October 2018 to January 2021 were selected as the research objects,including 38 males and 15 females,with an age of(47.95±7.16)years old,and 14 patients with normal intestinal mucosal tissue were selected during the same period,including 10 males and 4 females,with an age of(49.05±7.31)years old.Specimens were collected to make tissue chips,the expressions of CD44 and CD166 were detected by immunohistochemistry,CD44 and CD166 positive cells were located by immunohistochemical staining,and the results of CD44 and CD166 staining were counted.The expressions of CD44 and CD166 in UC-IN and normal intestinal mucosa were compared,and the expressions of CD44 and CD166 in UC-IN patients with different lesion invasion depths and pathological grades were analyzed.Results The CD44 positive cells of 53 UC-IN patients showed small focal weakly positive staining along the basal side of the duct,and the CD166 positive cells showed yellow cytoplasmic staining along the basal side of the duct;14 cases of normal intestinal mucosal tissue were all negatively stained for CD44,CD166 was basically negatively stained,and some small focal light yellow cytoplasmic staining on the basal side of the ducts was seen.The expressions of CD44 and CD166 in UC-IN patients were significantly higher than those in patients with normal intestinal mucosal tissue(P<0.05).The expressions of CD44 and CD166 in UC-IN patients with lesion invasion into the serosal layer were significantly higher than those in UC-IN patients with lesion invasion into the muscle layer(both P<0.05);the expressions of CD44 and CD166 in UC-IN patients with medium pathological grade were lower than those in UC-IN patients with low pathological g

关 键 词：溃疡性结肠炎 上皮内瘤变 CD44 CD166 免疫组化法 

分 类 号：R574.62[医药卫生—消化系统]