丹参发酵液的体外抗氧化和美白功效评价  

Evaluation of anti-oxidation and whitening efficacy of salvia miltiorrhiza fermentation broth in vitro

作　　者：莫秋婷 李萌[1] 王冬冬[1] 张佳婵[1] 王昌涛[1,2] 赵丹[1] Qiuting Mo;Meng Li;Dongdong Wang;Jiachan Zhang;Changtao Wang;Dan Zhao(Key Laboratory of Plant Resources Research and Development,College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100083,China;Center for Advanced Innovation in Food Nutrition and Human Health,Beijing Technology and Business University,Beijing 100083,China)

机构地区：[1]北京工商大学化学与材料工程学院植物资源研究与开发重点实验室,北京100083 [2]北京工商大学食品营养与人类健康高精尖创新中心,北京100083

出　　处：《日用化学工业》2021年第10期981-989,共9页China Surfactant Detergent & Cosmetics

摘　　要：利用水提法和微生物发酵法制备丹参水提液和丹参发酵液,在生化水平、细胞水平、转录水平上探究二者抗氧化和美白能力。实验结果表明二者对1,1-二苯基-2-三硝基苯肼(DPPH)自由基均有良好的清除能力,丹参水提液清除率达到50%时的体积分数(IC_(50))为10.3%,丹参发酵液IC50值为2.9%,在相同体积分数下,丹参发酵液清除能力更佳。在细胞水平上,二者均表现出对过氧化氢诱导损伤细胞的保护作用,相对于损伤模型,细胞总抗氧化能力提高、丙二醛(MDA)含量和活性氧(ROS)含量降低、过氧化氢酶(CAT)活力提高。在转录水平上,相对于损伤模型,二者作用于细胞后CAT表达量均提高。在黑色素合成和酪氨酸酶活力抑制作用实验中,丹参水提液和丹参发酵液相对于空白对照均表现出良好的美白作用。总体而言,相对于丹参水提液,丹参发酵液在抗氧化功效和美白功效方面均表现更佳。Water extraction and microbial fermentation were used to prepare salvia miltiorrhiza water extract(SMWE)and salvia miltiorrhiza fermentation broth(SMFB).Their antioxidative and whitening abilities were studied at biochemical,cellular and transcriptional levels.The experimental results show that both have good scavenging ability to DPPH free radicals.The IC50 value of SMWE is 10.3%,and the IC_(50) value of SMFB is 2.9%.At the same volume fraction,the scavenging ability of SMFB is better.At the cellular level,both of them show protective effects against hydrogen peroxide-induced injury of cells.Compared with the injury model,the total antioxidant capacity of the cells is increased,the MDA content and ROS content are reduced,and the CAT activity is increased.At the transcription level,it is verified that CAT expression is both increased after the two acting on cells.In the experiments of melanin synthesis inhibition and tyrosinase activity inhibition,the SMWE and the SMFB both show good whitening effects compared with the blank control.In general,compared with SMWE,the SMFB is better in terms of antioxidative and whitening effects.

关 键 词：丹参 发酵 抗氧化 美白 

分 类 号：TQ658[化学工程—精细化工]