nfluence and mechanism of Xindakang on cardiac functions of isolated frog heart  

作　　者：KONG Xiu-mei JIANG Yi HAN Yi-wei ZHAO Qin 

机构地区：[1]Engineering Research Center of Tibetan Medicine Testing Technology of Ministry of Education,Tibetan Medicine Screening Laboratory,School of Medicine,Tibet University for Nationalities,Xianyang 712082,China

出　　处：《中国药理学与毒理学杂志》2021年第10期764-765,共2页Chinese Journal of Pharmacology and Toxicology

基　　金：Natural Science Foundation of Tibet Autonomous Region(2015ZR-13-16)。

摘　　要：OBJECTIVE To evaluate the effects of flavonoids from Xindakang(Hippophae Fructus flavone)on myocardial systolic and diastolic functions of isolated frog hearts and explore the possible mechanism,and provide experimental basis for improving the effect and efficacy of Xindakang on cardiac function.METHODS The isolated frog heart perfusion specimens were prepared by Yagi′s method,and the effects of different concentrations of Xindakang on myocardial contractility(0.0125,0.025,0.05,0.1 and 0.2 g·L^(-1)),heart rate and cardiac output of isolated frog heart were studied.Acetylcholine,atropine and epinephrine were administered successively to analyze the effects of Xindakang on cardiac systolic function of isolated frogs under the action of different drugs,and compared with propranolol.The effect of extracellular calcium ion concentration on the action of Xindakang was studied by using low calcium concentration,high calcium concentration and normal Ren′s solution.To study the effect and possible mechanism of Xindakang on cardiac systolic function of frog.RESULTS The concentration of Xindakang in the range of 0.0125-0.1 g·L^(-1)could weaken the contractility of isolated frog heart and increase the concentration of Xindakang.The inhibitory effect of Xindakang on contractility of isolated frog heart was enhanced,and showed obvious dose-effect relationship.Cardiac output was significantly decreased by Xindakang(P<0.01),slow heart rate(P<0.05);M receptor blocker atropine could not antagonize the contractile effect of Xindakang,and Xindakang could not completely antagonize the contractile effect of adrenalin.Xindakang could inhibit the isolated frog heart in low calcium concentration,high calcium concentration and normal Ren′s solution,and increased with the increase of extracellular calcium concentration(P<0.01).CONCLUSION Xindakang has inhibitory effect on isolated frog heart,which may be achieved by blocking the calcium channel on myocardial cell membrane and reducing the calcium concentration in myocardial cells.

关 键 词：Hippophae Fructus FLAVONE Xindakang isolated frog heart cardiac function 

分 类 号：R965[医药卫生—药理学]