急性冠脉综合征不同气血证候亚型病人miRNAs表达谱检测及生物信息学分析  

Detection and Bioinformatics Analysis of miRNAs Expression Profiles in Patients with Different Qi-Blood Syndromes of Acute Coronary Syndrome

作　　者：赵烨婧 彭红玉 刘建勋[2] 柳景华 ZHAO Yejing;PENG Hongyu;LIU Jianxun;LIU Jinghua(Beijing Anzhen Hospital,Capital Medical University,Beijing Institute of Heart,Lung and Blood Vessel Diseases,Beijing 100029,China)

机构地区：[1]首都医科大学附属北京安贞医院北京市心肺血管疾病研究所,北京100029 [2]中国中医科学院西苑医院

出　　处：《中西医结合心脑血管病杂志》2021年第20期3448-3454,共7页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金项目(No.81970291);国家重点基础研究发展计划(973计划)课题(No.2015CB554404)。

摘　　要：目的利用基因表达芯片技术筛选气滞血瘀证和气虚血瘀证急性冠脉综合征病人差异微小RNAs(miRNAs)表达谱及相关功能通路,旨在探讨冠心病不同亚型血瘀证的潜在病理生理机制。方法筛选气滞血瘀证和气虚血瘀证病人外周血血清差异miRNAs,筛选标准为上调或下调倍数变化值≥2.0且P≤0.05,并对差异miRNAs进行靶基因预测和靶基因功能富集分析[基因本体(GO)分析和京都基因与基因组百科全书(KEGG)分析]。结果通过对气滞血瘀证组和气虚血瘀证组血清miRNAs表达微阵列经扫描和数据分析,共筛选出13个差异表达的miRNAs,包括11个表达上调的miRNAs(miR-7111-3p、miR-4515、miR-4436b-5p、miR-6743-3p、miR-4664-3p、miR-7974、miR-4646-3p、miR-6760-3p、miR-6507-3p、miR-6731-3p、miR-7114-3p)和2个表达下调的miRNAs(miR-3656、miR-4442)。功能分析发现预测的差异靶基因主要参与生物过程调控、代谢调控、信号转导调控等过程,并在磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路、Wnt信号通路、Apelin信号通路富集。结论共筛选出气虚血瘀证和气滞血瘀证病人13个差异表达的miRNAs,可能参与急性冠脉综合征气血失和的发生发展,同时PI3K/Akt等信号通路下调可能参与气虚血瘀证冠心病病理生理机制的形成。Objective To screen differential microRNAs(miRNAs)expression profiles and functional pathways between patients with Qi stagnation and blood stasis and Qi deficiency and blood stasis by gene expression chip technology and to explore the underlying pathophysiological mechanism of different subtypes of blood stasis syndrome.Methods Differential miRNAs expression profiles in the peripheral blood of the subjects between Qi stagnation and blood stasis group and Qi deficiency and blood stasis group were screened.The screening criteria were up-regulation or down-regulation multiple change value≥2.0 and P≤0.05,and the differential miRNA target gene prediction and target gene function enrichment analysis[Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis]were conducted.Results A total of 13 differentially expressed miRNAs were screened out,including 11 up-regulated miRNAs(miR-7111-3p,miR-4515,miR-4436b-5p,miR-6743-3p,miR-4664-3p,miR-7974,miR-4646-3p,miR-6760-3p,miR-6507-3p,miR-6731-3p,and miR-7114-3p)and 2 down-regulated miRNAs(miR-3656,miR-4442)by scanning and data analysis of serum miRNAs expression microarray in the Qi stagnation and blood stasis group and Qi deficiency and blood stasis group.Functional analysis showed that the predicted differential target genes were mainly involved in biological process regulation,metabolic regulation and signal transduction regulation,and there were significantly enriched in phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)signaling pathway,Wnt signaling pathway and Apelin signaling pathway.Conclusion A total of 13 miRNAs with differential expression were screened out from patients with Qi stagnation and blood stasis syndrome and Qi deficiency and blood stasis syndrome,which might play an important role in the development of the disturbance of Qi and blood discontinuity in acute coronary syndrome.The down-regulation of PI3K/Akt signaling pathway might be involved in the pathophysiological mechanism of coronary heart disease with Qi deficien

关 键 词：急性冠脉综合征 冠心病 气滞血瘀证 气虚血瘀证 微小RNAS 生物信息学分析 

分 类 号：R73[医药卫生—肿瘤]