灵芝多糖对B16F10黑素瘤细胞产生白介素10的影响  

Effect of Ganoderma Lucidum Polysaccharide on Interleukin-10 Production of B16F10 Melanoma Cells

作　　者：孙博 孙宇[1] 车姣子 李雪飞[1] 陆洁[1] 段昕所[1] SUN Bo;SUN Yu;CHE Jiao-zi;LI Xue-fei;LU Jie;DUAN Xin-suo(The Affi liated Hospital of Chengde Medical University,Chengde,Hebei,067000,China;The Central Hospital of Chengde)

机构地区：[1]承德医学院附属医院,河北承德067000 [2]承德市中心医院

出　　处：《承德医学院学报》2021年第5期371-374,共4页Journal of Chengde Medical University

摘　　要：目的探讨灵芝多糖对B16F10黑素瘤细胞产生白介素10(IL-10)的影响。方法分别将不同浓度灵芝多糖作用于B16F10黑素瘤细胞,通过RT-PCR方法和蛋白印迹(Western-blot)技术检测IL-10的表达情况。结果在不同浓度灵芝多糖作用下,经RT-PCR方法检测发现,加入0μg/mL、0.2μg/mL、0.8μg/mL、3.2μg/mL、12.8μg/mL灵芝多糖的B16F10黑素瘤细胞释放IL-10的量分别为1.03±0.15、1.15±0.25、0.92±0.11、0.86±0.64、0.75±0.12,与0μg/mL灵芝多糖组相比较,除0.2μg/mL灵芝多糖组,其他组差异均具有统计学意义,P<0.05。经Western-blot法检测,加入0μg/mL、0.2μg/mL、0.8μg/mL、3.2μg/mL、12.8μg/mL灵芝多糖的B16F10黑素瘤细胞IL-10表达量分别为1.47±0.43、1.58±0.31、0.93±0.12、0.89±0.26、0.40±0.29,与0μg/mL灵芝多糖组相比较,12.8μg/mL灵芝多糖组差异有统计学意义,P<0.05。结论灵芝多糖对B16F10黑素瘤细胞产生IL-10具有抑制作用。Objective To study the effect of production of Interleukin-10(IL-10)in the B16F10 cells by Ganoderma lucidum polysaccharides.Methods The B16F10 cell was prepared and treated with different concentrations of Ganoderma lucidum.The expression levels of IL-10 were assessed by Western-blot,RT-PCR.Results Under Ganoderma lucidum polysaccharides at different concentrations,RT-PCR revealed that the amount of IL-10 mRNA released by B16F10 melanoma cells treated with 0μg/mL,0.2μg/mL,0.8μg/mL,3.2μg/mL,12.8μg/mL was 1.03±0.15,1.15±0.25,0.92±0.11,0.86±0.64 and 0.75±0.12,respectively.Compared with the 0μg/mL Ganoderma lucidum polysaccharide group,the other groups showed statistical signifi cance except the 0.2μg/mL Ganoderma lucidum polysaccharide group,P<0.05.Westernblot revealed that the amount of IL-10 produced by B16F10 melanoma cell treated with 0μg/mL,0.2μg/mL,0.8μg/mL,3.2μg/mL,12.8μg/mL was 1.47±0.43,1.58±0.31,0.93±0.12,0.89±0.26 and 0.40±0.29,respectively.Compared with the 0μg/mL Ganoderma lucidum polysaccharide group,the 12.8μg/mL Ganoderma lucidum polysaccharide group showed statistical significance,P<0.05.Conclusion The production of IL-10 in the B16F10 cells was inhibited by Ganoderma lucidum polysaccharides.

关 键 词：B16F10黑素瘤细胞 灵芝多糖 IL-10 RT-PCR 蛋白印迹技术 

分 类 号：R739.5[医药卫生—肿瘤]