淫羊藿素对人卵巢癌A2780细胞增殖、凋亡、迁移和侵袭的影响  

Effect of Icaritin on Proliferation,Apoptosis,Migration and Invasion of Human Ovarian Cancer A2780 Cells

作　　者：李秋瑞 侯科名 王猛 陈蓉[1] LI Qiu-rui;HOU Ke-ming;WANG Meng;CHEN Rong(Chongqing Key Laboratory of Traditional Chinese Medicine(TCM)for Prevention and Cure of Metabolic Diseases,College of TCM,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学中医药学院,中医药防治代谢性疾病重庆市重点实验室,重庆400016

出　　处：《中国实验方剂学杂志》2021年第20期101-107,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市科委基金项目(cstc2016shmszx130057);重庆医科大学杏林计划-青苗工程资助项目(2021-ZDXK-M04)。

摘　　要：目的:观察淫羊藿素对人上皮性卵巢癌A2780细胞增殖、凋亡、迁移和侵袭的影响,并探讨淫羊藿素通过调控A2780细胞上皮间质转化(EMT)相关分子表达抑制细胞侵袭迁移。方法:设置空白组,淫羊藿素低、中、高剂量组(5,10,20μmol·L^(-1))分别作用于人卵巢癌A2780细胞48 h。采用细胞增殖与活性检测CCK-8)法,流式细胞术检测各组细胞增殖抑制率及凋亡率;划痕实验及transwell迁移实验观察细胞迁移情况并计算划痕愈合率与迁移率;transwell侵袭实验观察细胞侵袭情况并计算侵袭率;蛋白免疫印迹法(Western blot)与实时荧光定量聚合酶链式反应(Real-time PCR)检测EMT相关分子E-钙黏蛋白(E-cadherin),N-钙黏蛋白(N-cadherin),波形蛋白(Vimentin)和肿瘤侵袭迁移相关分子基质金属蛋白酶-9(MMP-9)蛋白及mRNA表达情况。结果:CCK-8与流式细胞术结果显示,与空白组比较,淫羊藿素各组细胞抑制率显著上升(P<0.01),细胞总凋亡率上升(P<0.05);划痕实验及transwell侵袭迁移实验结果显示,与空白组比较,淫羊藿素组的侵袭迁移能力减弱,细胞侵袭迁移数量和侵袭迁移率明显减少(P<0.05);Western blot与PCR结果显示,与空白组比较,淫羊藿素各组中N-cadherin,MMP-9,Vimentin蛋白和mRNA表达总体呈下降趋势,E-cadherin的表达呈上升趋势。结论:淫羊藿素对人卵巢癌A2780细胞具有抑制增殖、促进凋亡的作用,可能通过调控EMT相关分子表达抑制A2780细胞的侵袭迁移。Objective: To study the effect of icaritin on the proliferation,apoptosis,migration and invasion of human epithelial ovarian cancer A2780 cells and the inhibitory mechanism of icaritin against cell invasion and migration via the regulation of epithelial-mesenchymal transformation(EMT)-related molecule expression. Method:A2780 cells were divided into the blank control group and low-,medium-,and high-dose(5,10,20 μmol·L^(-1))icaritin groups and received the corresponding inventions for 48 h. Cell proliferation and viability were detected using the cell counting kit-8(CCK-8). The cellular proliferation inhibition and apoptosis rates were assayed by flow cytometry. The cell invasion and migration were observed in Scratch test and transwell test,followed by the calculation of wound healing rate and migration rate. The protein and mRNA expression levels of EMT-related molecules including E-cadherin,N-cadherin,and Vimentin and tumor invasion and migration-related molecule matrix metalloproteinase-9(MMP-9)were measured by Western blot and realtime polymerase chain reaction(Real-time PCR). Result: As revealed by CCK-8 assay and flow cytometry,compared with the blank control group,the icaritin groups all exhibited elevated proliferation inhibition rate(P<0.01)and apoptosis rate(P<0.05). According to the Scratch test and transwell test,compared with the blank control group,the icaritin groups displayed weakened invasion and migration ability and decreased number and rate of cell invasion and migration(P<0.05). Western blot and Real-time PCR results showed that the protein and mRNA expression levels of N-cadherin,MMP-9 and Vimentin in each icaritin group were down-regulated as compared with those in the blank control group, while the expression of E-cadherin was up-regulated.Conclusion: Icaritin inhibits the proliferation and promotes the apoptosis of human ovarian cancer A2780 cells,and it inhibits the invasion and migration of A2780 cells possibly by regulating the expression of EMT-related molecules.

关 键 词：淫羊藿素 卵巢癌A2780 上皮间质转化(EMT) 侵袭迁移 增殖凋亡 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]