大鼠肝纤维化相关microRNA及其候选靶基因的筛选  

Screening of Rat Liver Fibrosis Related MicroRNA and Its Candidate Target Genes

作　　者：侯晓丽 苏静慧 刘章心怡 张荣花 王一同 刘雨潭 张海东 章广玲 Hou Xiaoli;Su Jinghui;Liu Zhangxinyi;Zhang Ronghua;Wang Yitong;Liu Yutan;Zhang Haidong;Zhang Guangling(Clinical School of Medicine,North China University of Science and Technology,Tangshan,063000;Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases,Hebei Key Laboratory for Chronic Diseases,School of Basic Medical Sciences,North China University of Science and Technology,Tangshan,063000;Xiangya School of Medicine,Central South University,Changsha,410013;Jitang College,North China University of Science and Technology,Tangshan,063000)

机构地区：[1]华北理工大学临床医学院,唐山063000 [2]华北理工大学基础医学院,河北省慢性疾病重点实验室,唐山市慢性病临床基础研究重点实验室,唐山063000 [3]中南大学湘雅医学院,长沙410013 [4]华北理工大学冀唐学院,唐山063000

出　　处：《基因组学与应用生物学》2021年第3期1383-1390,共8页Genomics and Applied Biology

基　　金：国家自然科学基金青年科学基金项目(81201281);河北省自然科学基金资助项目(H2013209180);河北省卫计委项目(20170896)共同资助。

摘　　要：microRNAs(miRNAs)是一类功能性非编码RNA,在多种生物过程中具有重要作用。然而,miRNA的表达模式、调控网络以及参与肝纤维化的miRNA仍有待阐明。为了探讨与肝纤维化相关的miRNA及其靶基因的功能,为临床肝纤维化治疗提供理论依据,本研究前期已采用胆管结扎法(BDL)建立大鼠胆汁淤积性肝纤维化模型。从大鼠肝脏中提取总RNA,应用基因芯片技术对胆汁淤积性肝纤维化肝组织中miRNA和mRNA表达谱进行综合分析;结合生物信息方法分析在胆汁淤积性肝纤维化中差异表达miRNA可能的靶基因;实时荧光定量PCR技术检测TGF-β1处理人肝星状细胞LX-2细胞中miR-29a-3p、miR-194-5p和miR-22-3p相对表达水平。结果表明,与正常肝组织相比,纤维化肝组织中有48个差异表达miRNA(FC>2,P<0.05),其中36个上调,12个下调;筛选出18个预测靶基因参与与纤维化相关的生物过程;TGF-β1处理LX-2细胞中miR-29a-3p、miR-194-5p和miR-22-3p相对表达水平显著下调(P<0.05)。本研究筛选的差异表达miRNAs通过调节靶基因的表达在肝纤维化中可能发挥重要作用,将为miRNA在肝纤维化中的作用提供新的见解。MicroRNAs(miRNAs)are functional non-coding RNAs that play an important role in many biological processes.However,the expression patterns and regulatory networks,as well as the miRNAs involved in liver fibrosis remain to be elucidated.In order to investigate the function of miRNAs related to liver fibrosis and their target genes,and to provide theoretical basis for clinical treatment of liver fibrosis,bile duct ligation(BDL)was used to establish the rat cholestatic liver fibrosis model in the early stage of this study.Total RNA was extracted from rat liver.The expression profiles of miRNA and mRNA in cholestatic liver fibrosis were analyzed by gene chip technique.The possible target genes of miRNA differentially expressed in cholestatic liver fibrosis were analyzed by combining biological information method.The relative expression levels of miR-29a-3p,miR-194-5p and miR-22-3p in LX-2 cells treated by TGF-β1 were detected by quantitative real-time PCR.The results showed that,compared with normal liver tissues,there were 48 differentially expressed miRNAs(FC>2,P<0.05)in fibrosis liver tissues,among which 36 were up-regulated and 12 were down-regulated.Eighteen target genes were selected to participate in biological processes related to fibrosis.The relative expression levels of miR-29a-3p,miR-194-5p and miR-22-3p in Lx-2 cells treated by TGF-β1 were significantly down-regulated(P<0.05).The differentially expressed miRNAs screened in this study may play an important role in liver fibrosis by regulating the expression of target genes,which will provide new insights into the role of miRNA in liver fibrosis.

关 键 词：胆汁淤积 肝纤维化 MIRNA MRNA 

分 类 号：R575.2[医药卫生—消化系统]