异麦角甾苷对肺癌细胞A549凋亡和氧化应激的影响  

Effect of Isoacteoside on Apoptosis and Oxidative Stress of Lung Cancer Cell Line A549

作　　者：李奎 谢颂平[2] 江万里[2] 范国华[2] LI Kui;XIE Songping;JIANG Wanli;FAN Guohua(Department of Cardiothoracic Surgery,Yiling Hospital,Yichang,Hubei,443100,China;Department of Thoracic Surgery,People’s Hospital of Wuhan University,Wuhan,430060,China)

机构地区：[1]宜昌市夷陵医院胸心血管外科湖北省宜昌市,443100 [2]武汉大学人民医院胸外科武汉市,430060

出　　处：《医学分子生物学杂志》2021年第5期338-342,348,共6页Journal of Medical Molecular Biology

基　　金：国家自然科学基金(No.81801954)。

摘　　要：目的探讨异麦角甾苷(ISO)对A549细胞凋亡和氧化应激的影响。方法CCK-8实验检测细胞的活力;A549细胞分为ISO 0、5、10和20μmol/L 4组,克隆形成实验检测细胞增殖;流式细胞术检测细胞凋亡和线粒体膜电位变化;Western印迹检测凋亡相关蛋白的表达;试剂盒检测细胞中的超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果与ISO 0μmol/L组比较,ISO 10μmol/L及以上浓度均显著降低A549细胞活力(P<0.05),ISO 10和20μmol/L组抑制A549细胞的增殖(P<0.05),促进细胞的凋亡(P<0.05),降低细胞线粒体膜电位,上调Bax/Bcl-2和Caspase-3蛋白表达,下调c-Myc蛋白表达(P<0.05),促进氧化应激(P<0.05)。结论ISO能抑制A549细胞增殖,促进细胞凋亡和氧化应激。Objective To investigate the effect of isoacteoside(ISO)on the apoptosis and oxidative stress of A549 cells.Methods CCK-8 assay was used to detect cell viability.A549 cells were divided into 4 groups in terms of ISO concentrations(0,5,10 and 20μmol/L),and cell proliferation was detected by clone formation experiment.Apoptosis and mitochondrial membrane potential were detected by flow cytometry.The expression of apoptosis-related proteins was detected by Western blotting.The contents of superoxide dismutase(SOD)and malondialdehyde(MDA)were measured in cells.Results ISO at 10μmol/L and above could significantly decrease the viability of A549 cells(P<0.05).ISO at 10 and 20μmol/L could significantly inhibit the proliferation of A549 cells(P<0.05),promote apoptosis(P<0.05),decrease mitochondrial membrane potential,up-regulate the expression of Bax/Bcl-2 and Caspase-3 protein,down-regulate the expression of c-Myc protein,and promote oxidative stress(P<0.05).Conclusion ISO can inhibit the proliferation of A549 cells,and promote apoptosis and oxidative stress.

关 键 词：异麦角甾苷 肺癌 凋亡 BAX 氧化应激 

分 类 号：R734.2[医药卫生—肿瘤]