阿嗪米特对质子泵抑制剂相关低胃酸环境下胃排空的影响  

Effects of azintamide on gastric emptying in proton pump inhibitor related low gastric acid environment

作　　者：韩涛涛 陈楚岩 王静 李景南 Han Taotao;Chen Chuyan;Wang Jing;Li Jingnan(Department of Gastroenterology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Key Laboratory of Intestinal Microecology and Clinical Transformation,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]中国医学科学院,北京协和医学院,北京协和医院消化内科,中国医学科学院肠道微生态与临床转化研究重点实验室,100730

出　　处：《中华消化杂志》2021年第9期599-605,共7页Chinese Journal of Digestion

基　　金：中国医学科学院医学与健康科技创新工程(2016-I2M-1-007)。

摘　　要：目的研究阿嗪米特在质子泵抑制剂相关低胃酸环境中对胃排空能力和胃肠激素分泌的影响及其潜在机制。方法选取60只大鼠,采用随机数字表法将大鼠分为低胃酸对照组、低胃酸模型组、阿嗪米特+低胃酸模型组、高胃酸对照组、高胃酸模型组和阿嗪米特+高胃酸模型组,每组10只。其中低胃酸和高胃酸对照组大鼠均以0.9%氯化钠溶液进行处理,低胃酸模型组和高胃酸模型组大鼠分别采用奥美拉唑(20 mg/kg,腹腔注射,1次/d,连续7 d)和五肽胃泌素(2 mg/kg,皮下注射,1次/d,连续3 d)构建模型,阿嗪米特+低胃酸模型组和阿嗪米特+高胃酸模型组大鼠分别在低胃酸和高胃酸模型基础上给予阿嗪米特(50 mg/kg,灌胃,连续3 d)进行干预。以下仅分析构建低胃酸模型的3组大鼠的情况:建模第0、2、4、6、8天,测量并比较大鼠体重;建模结束后,测定并比较大鼠胃内容物质量、胃液pH值,采用酶联免疫吸附测定法检测并比较大鼠外周血胃蛋白酶原A、胃泌素和胆囊收缩素水平。统计学方法采用单因素方差分析和Tukey忠实显著性差异法。结果低胃酸模型组和低胃酸+阿嗪米特干预组大鼠胃液的pH值均高于低胃酸对照组(2.17±0.53、2.03±0.69比1.32±0.17),差异均有统计学意义(P=0.026、0.041);低胃酸模型组与低胃酸+阿嗪米特干预组大鼠胃液的pH值比较差异无统计学意义(P>0.05)。低胃酸对照组、低胃酸模型组、低胃酸+阿嗪米特干预组大鼠在建模第0、2、4、6、8天的体重分别为(285.40±10.86)、(283.40±6.38)、(282.00±5.04)g,(287.10±10.73)、(283.20±5.83)、(284.00±5.72)g,(292.20±11.18)、(281.90±6.23)、(289.00±5.82)g,(296.40±11.12)、(277.70±6.96)、(292.00±6.82)g,(300.80±11.29)、(274.30±8.84)、(297.00±4.17)g。低胃酸模型组大鼠在建模第6、8天的体重均低于低胃酸对照组,低胃酸+阿嗪米特干预组大鼠在建模第6、8天的体重均高于低胃酸模型组,差Objective To explore the effects and underlying mechanisms of azintamide on gastric emptying and gastrointestinal hormone secretion in proton pump inhibitor related low gastric acid environment.Methods A total of 60 rats were selected and randomly divided into low gastric acid control group,low gastric acid model group,low gastric acid and azintamide intervention group,high gastric acid control group,high gastric acid model group and high gastric acid and azintamide intervention group by random number table,with 10 rats in each group.The rats of low gastric acid control group and high gastric acid control group were all treated with 0.9%sodium chloride solution.The rats of low gastric acid model group and high gastric acid model group were established by intraperitoneal injection of 20 mg/kg omeprazole once per day for seven days,and subcutaneous injection of 2 mg/kg penta gastrin once per day for three days,respectively.The rats of low gastric acid and azintamide intervention group and high gastric acid and azintamide intervention group were gavaged with azintamide 50 mg/kg once per day for three days on the basis of low gastric acid model group and high gastric acid model group,respectively.Only the rats in three low gastric acid groups were analyzed.At Day 0,2nd,4th,6th and 8th after modeling,the body weight of rats were compared.After modeling,the weight of gastric contents and pH of gastric fluid was measured and compared,and the peripheral blood levels of pepsinogen A(PGA),gastrin and cholecystokinin(CCK)were detected by enzyme linked immunosorbent assay.One-way analysis of variance and Tukey′s honestly significant difference post-hoc test were used for statistical analysis.Results The pH value of gastric fluid in low gastric acid model group and low gastric acid and azintamide intervention group were both higher than that in the low gastric acid control group(2.17±0.53,2.03±0.69 vs.1.32±0.17),and the differences were statistically significant(P=0.026 and 0.041,respectively).While there was no signific

关 键 词：阿嗪米特 质子泵抑制剂 胆囊收缩素 胃泌素类 胃蛋白酶原A 

分 类 号：R57[医药卫生—消化系统]